University of Bern, Department of Restorative, Preventive and Pediatric Dentistry, School of Dental Medicine, Bern, Switzerland.
Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
J Appl Oral Sci. 2023 Jul 24;31:e20230108. doi: 10.1590/1678-7757-2023-0108. eCollection 2023.
Polyphenols interact with salivary proteins and thus can improve the pellicle's erosion protective properties. This effect could be exploited to create rinsing solutions with polyphenols as active ingredients for erosion prevention. Different from the current gold standard for erosion protective rinsing solutions, these rinses would not rely on stannous ions. This would offer alternatives for patients with concerns regarding the composition of rinsing solutions and preferring bio-products.
To develop an erosion-preventive rinsing solution containing natural polyphenol-rich extracts.
Solutions were prepared with polyphenols from either grapeseed extract or cranberry extract, 500 ppm fluoride added, and additionally flavors and sweeteners. Controls were deionized water, 500 ppm fluoride solution, and the gold standard rinse in the field (Sn2+/F-). In total, 135 enamel specimens (n=15/group) were subjected to five cycles of salivary pellicle formation (30 min, 37°C), modification with the solutions (2 min, 25°C), further salivary pellicle formation (60 min, 37°C), and erosive challenge (1 min, 1% citric acid, pH 3.6). Relative surface microhardness (rSMH), surface reflection intensity (rSRI), and amount of calcium release (CaR) were investigated. Data were analyzed with Kruskal-Wallis and Wilcoxon rank sum tests (α=0.05).
The polyphenol solutions containing fluoride, as well as additional flavors, protected enamel better than fluoride alone, and similar to the Sn2+/F- solution, when investigating both rSMH and CaR. When measuring rSRI, Sn2+/F- showed the best protection, while the polyphenol solutions were similar to fluoride.
For two of the three assessed parameters (rSMH and CaR), both developed polyphenol-rich rinsing solutions were able to protect enamel from erosion, improving/potentializing the effect of fluoride and matching the protection offered by the current gold standard rinsing solution.
多酚与唾液蛋白相互作用，从而可以改善牙菌斑的抗侵蚀保护性能。可以利用这种效果来开发含有多酚作为抗侵蚀活性成分的漱口液。与目前抗侵蚀漱口液的金标准不同，这些漱口液不会依赖于锡离子。这将为那些对漱口液成分有顾虑、更喜欢生物制品的患者提供替代方案。
开发一种含有天然多酚丰富提取物的抗侵蚀漱口液。
用葡萄籽提取物或蔓越莓提取物中的多酚、添加 500ppm 氟化物以及口味剂和甜味剂制备溶液。对照组为去离子水、500ppm 氟化物溶液和该领域的金标准漱口液（Sn2+/F-）。共对 135 个牙釉质样本（n=15/组）进行了 5 个唾液膜形成周期（30min，37°C）、用溶液处理（2min，25°C）、进一步唾液膜形成（60min，37°C）和侵蚀挑战（1min，1%柠檬酸，pH 值 3.6）。研究了相对表面显微硬度（rSMH）、表面反射强度（rSRI）和钙释放量（CaR）。数据采用 Kruskal-Wallis 和 Wilcoxon 秩和检验（α=0.05）进行分析。
含有氟化物和额外口味剂的多酚溶液在 rSMH 和 CaR 两个评估参数中都比单独的氟化物更能保护牙釉质，与 Sn2+/F-溶液相似。在测量 rSRI 时，Sn2+/F-显示出最佳的保护效果，而多酚溶液与氟化物相似。
在三个评估参数中的两个（rSMH 和 CaR）中，两种开发的富含多酚的漱口液都能够保护牙釉质免受侵蚀，增强了氟化物的效果，与当前的金标准漱口液提供的保护效果相当。
