Eo Hyun Ji, Jang Jun Hyuk, Park Gwang Hun
Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea.
Plants (Basel). 2021 Mar 19;10(3):586. doi: 10.3390/plants10030586.
(Wall.) Brongn. (BF), which belongs to Rhamnaceae, is a special plant of Anmyeon Island in Korea. BF has been reported to have antioxidant and whitening effects. However, the anti-inflammatory activity of BR has not been elucidated. In this study, we evaluated the anti-inflammatory effect of leaves (BR-L), branches (BR-B) and fruit (BR-F) extracted with 70% ethanol of BR and elucidated the potential signaling pathway in LPS-induced RAW264.7 cells. BR-L showed a strong anti-inflammatory activity through the inhibition of NO production. BR-L significantly suppressed the production of the pro-inflammatory mediators such as iNOS, COX-2, IL-1β, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells. BR-L suppressed the degradation and phosphorylation of IκB-α, which contributed to the inhibition of p65 nuclear accumulation and NF-κB activation. BR-L obstructed the phosphorylation of MAPKs (ERK1/2, p38 and JNK) in LPS-stimulated RAW264.7 cells. Consequently, these results suggest that BR-L may have great potential for the development of anti-inflammatory drugs to treat acute and chronic inflammatory disorders.
（Wall.）Brongn.（BF）属于鼠李科，是韩国安眠岛的一种特殊植物。据报道，BF具有抗氧化和美白作用。然而，BR的抗炎活性尚未阐明。在本研究中，我们评估了用70%乙醇提取的BR的叶（BR-L）、枝（BR-B）和果实（BR-F）的抗炎作用，并阐明了其在LPS诱导的RAW264.7细胞中的潜在信号通路。BR-L通过抑制NO生成表现出强大的抗炎活性。BR-L显著抑制LPS刺激的RAW264.7细胞中促炎介质如诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的产生。BR-L抑制IκB-α的降解和磷酸化，这有助于抑制p65核积累和核因子-κB（NF-κB）激活。BR-L阻碍LPS刺激的RAW264.7细胞中丝裂原活化蛋白激酶（MAPKs，细胞外信号调节激酶1/2（ERK1/2）、p38和应激活化蛋白激酶（JNK））的磷酸化。因此，这些结果表明，BR-L在开发用于治疗急慢性炎症性疾病的抗炎药物方面可能具有巨大潜力。
