Department of Science, University of Basilicata, 85100 Potenza, Italy.
Teagasc Food Research Centre, Ashtown, Dublin D15KN3K, Ireland.
Molecules. 2018 Sep 29;23(10):2497. doi: 10.3390/molecules23102497.
Antioxidant phytochemicals play a key role in oxidative stress control and in the prevention of related disorders, such as premature aging, degenerative diseases, diabetes, and cancer. The aim of this study was to investigate the potential antioxidant activity and the phytochemical profile of Wedd., a perennial shrub, belonging to the Asteraceae family. Despite the wide interest of this family, this specie has not been investigated yet. aerial parts were extracted with 96% ethanol. Then, the ethanol extract was fractionated by liquid/liquid extraction using an increasing solvents polarity. Total polyphenol and terpenoid contents were measured. Moreover, the antioxidant activity was evaluated by six different complementary in vitro assays. The Relative Antioxidant Capacity Index (RACI) was used to compare data obtained by different tests. The sample showing the highest RACI was subjected to characterization and quantitation of its phenolic composition using LC-MS/MS analysis. The ethyl acetate fraction, investigated by LC-MS/MS analysis, showed 30 compounds, most of them are chlorogenic acid and flavonoid derivatives. To the best of our knowledge, this is the first report about the evaluation of antioxidant activity and phytochemical profile of , underlying the importance of this species as a source of health-promoting phytochemicals.
抗氧化植物化学物质在控制氧化应激和预防相关疾病（如早衰、退行性疾病、糖尿病和癌症）方面发挥着关键作用。本研究旨在研究属于菊科的多年生灌木 Wedd. 的潜在抗氧化活性和植物化学特征。尽管该家族受到广泛关注，但尚未对该物种进行研究。用 96%乙醇提取地上部分。然后，用液/液萃取法用增加的溶剂极性对乙醇提取物进行分级。测定总多酚和萜烯含量。此外，通过六种不同的体外互补测定来评估抗氧化活性。相对抗氧化能力指数（RACI）用于比较不同测试获得的数据。显示出最高 RACI 的样品用 LC-MS/MS 分析进行表征和定量其酚类成分。通过 LC-MS/MS 分析研究的乙酸乙酯馏分显示出 30 种化合物，其中大多数是绿原酸和类黄酮衍生物。据我们所知，这是首次报道关于评估抗氧化活性和植物化学特征的研究，这突显了该物种作为具有促进健康的植物化学物质来源的重要性。
