Dipartimento di Scienze Chimiche, Università degli Studi di Catania, Viale A. Doria 6, 95125 Catania, Italy.
Istituto di Cristallografia, CNR, via P. Gaifami 18, 95126 Catania, Italy.
Molecules. 2021 Mar 19;26(6):1724. doi: 10.3390/molecules26061724.
Over the years, cyclodextrin uses have been widely reviewed and their proprieties provide a very attractive approach in different biomedical applications. Cyclodextrins, due to their characteristics, are used to transport drugs and have also been studied as molecular chaperones with potential application in protein misfolding diseases. In this study, we designed cyclodextrin polymers containing different contents of β- or γ-cyclodextrin, and a different number of guanidinium positive charges. This allowed exploration of the influence of the charge in delivering a drug and the effect in the protein anti-aggregant ability. The polymers inhibit Amiloid β peptide aggregation; such an ability is modulated by both the type of CyD cavity and the number of charges. We also explored the effect of the new polymers as drug carriers. We tested the Doxorubicin toxicity in different cell lines, A2780, A549, MDA-MB-231 in the presence of the polymers. Data show that the polymers based on γ-cyclodextrin modified the cytotoxicity of doxorubicin in the A2780 cell line.
多年来，环糊精的用途得到了广泛的综述，其特性为不同的生物医学应用提供了极具吸引力的方法。由于其特性，环糊精被用于输送药物，并且还被研究作为分子伴侣，在蛋白质错误折叠疾病中有潜在的应用。在这项研究中，我们设计了含有不同含量β-或γ-环糊精和不同数量胍正电荷的环糊精聚合物。这允许探索在输送药物时电荷的影响以及对蛋白质抗聚集能力的影响。聚合物抑制淀粉样β肽聚集；这种能力既受 CyD 腔的类型又受电荷数的调节。我们还探索了新聚合物作为药物载体的效果。我们在存在聚合物的情况下，在不同的细胞系 A2780、A549 和 MDA-MB-231 中测试了阿霉素的毒性。数据表明，基于 γ-环糊精的聚合物改变了阿霉素在 A2780 细胞系中的细胞毒性。
