Alvarez-Mon Miguel Angel, Gómez-Lahoz Ana Maria, Orozco Arancha, Lahera Guillermo, Diaz David, Ortega Miguel A, Albillos Agustin, Quintero Javier, Aubá Enrique, Monserrat Jorge, Alvarez-Mon Melchor
Department of Psychiatry and Medical Psychology, University Clinic of Navarra, Avda. Pío XII, 36, 31008 Pamplona, Spain.
Department of Medicine and Medical Specialities, University of Alcala, 28871 Alcala de Henares, Spain.
J Pers Med. 2021 Mar 19;11(3):220. doi: 10.3390/jpm11030220.
We have investigated the distribution of the Th1, Th2 and Th17 subsets in circulating CD4 T lymphocytes and their naïve (T), effector (T), central (T) and effector memory (T) activation/differentiation stages in patients with major depressive disorder (MDD).
Thirty MDD patients and 30 healthy controls were studied. The counts of circulating CD4 T lymphocytes and their distribution on the T, T, T and T activation/differentiation stages were analyzed by polychromatic flow cytometry. The intracytoplasmic interferon gamma (IFNγ), interleukin (IL)-4, IL-17A and tumor necrosis factor alpha (TNF-alpha) and membrane CD28 expression were also measured. The serum IFNγ, IL-4, Il-17A and TNF-alpha were measured by Luminex, respectively.
MDD patients had normal counts of CD4 T lymphocytes and of their T, T and T subsets but increased number and percentage of T CD4 subset. CD4 T lymphocytes had significantly enhanced percentage of cells that express IL-17 and TNF-alpha explained by the expansions found in the T, T and, T and T, T and T activation/differentiation stages, respectively. A selective increase in the percentages of T and T expressing IFNγ was also observed. We found a significant correlation between the percentages of CD4 T lymphocytes expressing IFNγ and TNF-alpha in these patients. MDD patients showed increased serum levels of IL-17 and TNF-alpha, but normal IFNγ and IL-4 concentration.
the cross-sectional nature of the study could be considered a limitation.
MDD patients have abnormal circulating CD4 T lymphocytes with expansion of the IL-17 and TNF-alpha expressing cells as well as increased levels of circulating IL-17 and TNF-alpha.
我们研究了重度抑郁症（MDD）患者循环CD4 T淋巴细胞中Th1、Th2和Th17亚群的分布及其初始（T）、效应（T）、中枢（T）和效应记忆（T）激活/分化阶段。
对30例MDD患者和30名健康对照者进行研究。采用多色流式细胞术分析循环CD4 T淋巴细胞的计数及其在T、T、T和T激活/分化阶段的分布。同时检测细胞内干扰素γ（IFNγ）、白细胞介素（IL）-4、IL-17A和肿瘤坏死因子α（TNF-α）以及膜CD28的表达。分别采用Luminex法检测血清IFNγ、IL-4、Il-17A和TNF-α。
MDD患者CD4 T淋巴细胞及其T、T和T亚群的计数正常，但T CD4亚群的数量和百分比增加。CD4 T淋巴细胞表达IL-17和TNF-α的细胞百分比显著增加，分别由T、T以及T、T和T、T激活/分化阶段的扩增所解释。还观察到表达IFNγ的T和T百分比选择性增加。我们发现这些患者中表达IFNγ和TNF-α的CD4 T淋巴细胞百分比之间存在显著相关性。MDD患者血清IL-17和TNF-α水平升高，但IFNγ和IL-4浓度正常。
该研究的横断面性质可被视为一种局限性。
MDD患者循环CD4 T淋巴细胞异常，表达IL-17和TNF-α的细胞扩增，循环IL-17和TNF-α水平升高。
