Palau Montserrat, Piqué Núria, Ramírez-Lázaro M José, Lario Sergio, Calvet Xavier, Miñana-Galbis David
Secció de Microbiologia, Departament de Biologia, Sanitat i Medi Ambient, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Av. Joan XXIII 27-31, 08028 Barcelona, Catalonia, Spain.
Digestive Diseases Service, Hospital de Sabadell, Institut Universitari Parc Taulí-UAB, Parc Tauli 1, 08208 Sabadell, Catalonia, Spain.
Pathogens. 2021 Mar 12;10(3):331. doi: 10.3390/pathogens10030331.
is a common pathogen associated with several severe digestive diseases. Although multiple virulence factors have been described, it is still unclear the role of virulence factors on pathogenesis and disease progression. Whole genome sequencing could help to find genetic markers of virulence strains. In this work, we analyzed three complete genomes from isolates obtained at the same point in time from a stomach of a patient with adenocarcinoma, using multiple available bioinformatics tools. The genome analysis of the strains B508A-S1, B508A-T2A and B508A-T4 revealed that they were , and negative. The differences among the three genomes were mainly related to outer membrane proteins, methylases, restriction modification systems and flagellar biosynthesis proteins. The strain B508A-T2A was the only one presenting the genotype , and had the most distinct genome as it exhibited fewer shared genes, higher number of unique genes, and more polymorphisms were found in this genome. With all the accumulated information, no significant differences were found among the isolates regarding virulence and origin of the isolates. Nevertheless, some B508A-T2A genome characteristics could be linked to the pathogenicity of .
是一种与多种严重消化系统疾病相关的常见病原体。尽管已经描述了多种毒力因子，但毒力因子在发病机制和疾病进展中的作用仍不清楚。全基因组测序有助于找到毒力菌株的遗传标记。在这项工作中，我们使用多种可用的生物信息学工具，分析了从一名腺癌患者胃部同一时间点分离得到的三株菌的完整基因组。菌株B508A-S1、B508A-T2A和B508A-T4的基因组分析表明，它们分别为 、 和 阴性。这三个基因组之间的差异主要与外膜蛋白、甲基化酶、限制修饰系统和鞭毛生物合成蛋白有关。菌株B508A-T2A是唯一呈现基因型 的菌株，并且具有最独特的基因组，因为它显示出较少的共享基因、较多的独特基因，并且在该基因组中发现了更多的多态性。综合所有积累的信息，在分离株的毒力和来源方面未发现显著差异。然而，一些B508A-T2A基因组特征可能与 的致病性有关。
