Zhang Aidi, Zhou Hui, Jiang Xiaohan, Han Yuepeng, Zhang Xiujun
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430000, China.
Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan 430074, China.
Plants (Basel). 2021 Mar 12;10(3):538. doi: 10.3390/plants10030538.
The flat peach has become more and more popular worldwide for its fruit quality with relatively low acidity, high sugar content and rich flavor. However, the draft genome assembly of flat peach is still unavailable and the genetic basis for its fruit flavor remains unclear. In this study, the draft genome of a flat peach cultivar '124 Pan' was assembled by using a hybrid assembly algorithm. The final assembly resulted in a total size of 206 Mb with a N50 of 26.3 Mb containing eight chromosomes and seven scaffolds. Genome annotation revealed that a total of 25,233 protein-coding genes were predicted with comparable gene abundance among the sequenced peach species. The phylogenetic tree and divergence times inferred from 572 single copy genes of 13 plant species confirmed that was the ancestor of the domesticated peach. By comparing with the genomes of (Lovell) and , the expansion of genes encoding enzymes involved in terpene biosynthesis was found, which might contribute to the good fruit flavor traits of '124 Pan'. The flat peach draft genome assembly obtained in this study will provide a valuable genomic resource for peach improvement and molecular breeding.
蟠桃因其果实品质酸度相对较低、糖分含量高且风味浓郁,在全球越来越受欢迎。然而,蟠桃的基因组草图组装仍然不可用,其果实风味的遗传基础仍不清楚。在本研究中,利用混合组装算法组装了蟠桃品种‘124蟠’的基因组草图。最终组装结果的总大小为206 Mb,N50为26.3 Mb,包含8条染色体和7个支架。基因组注释显示,共预测到25233个蛋白质编码基因,在所测序的桃品种中基因丰度相当。从13种植物的572个单拷贝基因推断出的系统发育树和分歧时间证实,[此处原文缺失相关内容]是驯化桃的祖先。通过与[此处原文缺失相关品种](洛弗尔)和[此处原文缺失相关品种]的基因组进行比较,发现了参与萜类生物合成的酶编码基因有所扩增,这可能有助于‘124蟠’良好的果实风味特征。本研究获得的蟠桃基因组草图组装将为桃的改良和分子育种提供有价值的基因组资源。