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人硬膜外脂肪来源间充质干细胞与成纤维细胞的细胞外囊泡比较。

Comparisons of Extracellular Vesicles from Human Epidural Fat-Derived Mesenchymal Stem Cells and Fibroblast Cells.

机构信息

Department of Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, Korea.

Department of Biomaterials Science (BK21 Four Program), Life and Industry Convergence Institute, Pusan National University, Miryang 50463, Korea.

出版信息

Int J Mol Sci. 2021 Mar 12;22(6):2889. doi: 10.3390/ijms22062889.

DOI:10.3390/ijms22062889
PMID:33809214
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8000612/
Abstract

Extracellular vesicles (EVs) are generated and secreted by cells into the circulatory system. Stem cell-derived EVs have a therapeutic effect similar to that of stem cells and are considered an alternative method for cell therapy. Accordingly, research on the characteristics of EVs is emerging. EVs were isolated from human epidural fat-derived mesenchymal stem cells (MSCs) and human fibroblast culture media by ultracentrifugation. The characterization of EVs involved the typical evaluation of cluster of differentiation (CD antigens) marker expression by fluorescence-activated cell sorting, size analysis with dynamic laser scattering, and morphology analysis with transmission electron microscopy. Lastly, the secreted levels of cytokines and chemokines in EVs were determined by a cytokine assay. The isolated EVs had a typical size of approximately 30-200 nm, and the surface proteins CD9 and CD81 were expressed on human epidural fat MSCs and human fibroblast cells. The secreted levels of cytokines and chemokines were compared between human epidural fat MSC-derived EVs and human fibroblast-derived EVs. Human epidural fat MSC-derived EVs showed anti-inflammatory effects and promoted macrophage polarization. In this study, we demonstrated for the first time that human epidural fat MSC-derived EVs exhibit inflammatory suppressive potency relative to human fibroblast-derived EVs, which may be useful for the treatment of inflammation-related diseases.

摘要

细胞外囊泡 (EVs) 由细胞生成并分泌到循环系统中。干细胞来源的 EVs 具有与干细胞相似的治疗效果，被认为是细胞治疗的一种替代方法。因此，对 EVs 的特征的研究正在兴起。通过超速离心法从人硬膜外脂肪间充质干细胞 (MSCs) 和人成纤维细胞培养基中分离 EVs。EVs 的特征包括通过荧光激活细胞分选对分化簇 (CD) 抗原标志物表达的典型评估、通过动态激光散射进行的大小分析以及通过透射电子显微镜进行的形态分析。最后，通过细胞因子测定法确定 EVs 中细胞因子和趋化因子的分泌水平。分离的 EVs 具有约 30-200nm 的典型大小，并且 CD9 和 CD81 表面蛋白在人硬膜外脂肪 MSC 和人成纤维细胞上表达。比较了人硬膜外脂肪 MSC 衍生 EVs 和人成纤维细胞衍生 EVs 之间的细胞因子和趋化因子的分泌水平。人硬膜外脂肪 MSC 衍生的 EVs 显示出抗炎作用，并促进巨噬细胞极化。在这项研究中，我们首次证明人硬膜外脂肪 MSC 衍生的 EVs 相对于人成纤维细胞衍生的 EVs 具有炎症抑制作用，这可能对治疗炎症相关疾病有用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2922/8000612/c8b59912fab6/ijms-22-02889-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2922/8000612/412d7e26c172/ijms-22-02889-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2922/8000612/17784d062b65/ijms-22-02889-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2922/8000612/7629f2808933/ijms-22-02889-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2922/8000612/c8b59912fab6/ijms-22-02889-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2922/8000612/412d7e26c172/ijms-22-02889-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2922/8000612/17784d062b65/ijms-22-02889-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2922/8000612/7629f2808933/ijms-22-02889-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2922/8000612/c8b59912fab6/ijms-22-02889-g004.jpg

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