Dimopoulou Maria, Kefalloniti Vasiliki, Tsakanikas Panagiotis, Papanikolaou Seraphim, Nychas George-John E
Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, School of Food and Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
Microorganisms. 2021 Mar 12;9(3):587. doi: 10.3390/microorganisms9030587.
is a wine spoilage yeast known to colonize and persist in production cellars. However, knowledge on the biofilm formation capacity of remains limited. The present study investigated the biofilm formation of 11 strains on stainless steel coupons after 3 h of incubation in an aqueous solution. FTIR analysis was performed for both planktonic and attached cells, while comparison of the obtained spectra revealed chemical groups implicated in the biofilm formation process. The increased region corresponding to polysaccharides and lipids clearly discriminated the obtained spectra, while the absorption peaks at the specific wavenumbers possibly reveal the presence of β-glucans, mannas and ergosterol. Unsupervised clustering and supervised classification were employed to identify the important wavenumbers of the whole spectra. The fact that all the metabolic fingerprints of the attached versus the planktonic cells were similar within the same cell phenotype class and different between the two phenotypes, implies a clear separation of the cell phenotype; supported by the results of the developed classification model. This study represents the first to succeed at applying a non-invasive technique to reveal the metabolic fingerprint implicated in the biofilm formation capacity of underlying the homogenous mechanism within the yeast species.
是一种已知会在生产酒窖中定殖并持续存在的葡萄酒变质酵母。然而，关于其生物膜形成能力的知识仍然有限。本研究调查了11株该酵母菌株在水溶液中孵育3小时后在不锈钢试片上的生物膜形成情况。对浮游细胞和附着细胞都进行了傅里叶变换红外光谱（FTIR）分析，而对所得光谱的比较揭示了参与生物膜形成过程的化学基团。对应于多糖和脂质的增加区域清楚地区分了所得光谱，而特定波数处的吸收峰可能揭示了β-葡聚糖、甘露聚糖和麦角固醇的存在。采用无监督聚类和有监督分类来识别整个光谱的重要波数。在同一细胞表型类别中，附着细胞与浮游细胞的所有代谢指纹相似，而在两种表型之间不同，这一事实意味着细胞表型的明显分离；所开发分类模型的结果支持了这一点。本研究首次成功应用非侵入性技术揭示了该酵母生物膜形成能力所涉及的代谢指纹，其基于酵母物种内的同源机制。
