State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Department of Orthodontics, Stomatological Center, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, Guangdong.
Biosci Rep. 2019 Dec 20;39(12). doi: 10.1042/BSR20190785.
Stromal cell derived factor-1 (SDF-1) and basic fibroblast growth factor (bFGF) were reported to induce the differentiation of bone marrow stem cells (BMSCs) into cells with characteristics of periodontal ligament fibroblasts. Thus SDF-1 and bFGF may play a positive role in BMSCs-mediated periodontal ligament regeneration. Here, the methylthiazolyldiphenyl tetrazolium bromide (MTT) assay was used to investigate the effect of scaffolds, SDF-1 and bFGF on BMSCs proliferation. RT-PCR and Western blot were used to evaluate gene and protein expression. Beagle dogs were used to establish an animal model of tooth reimplantation and to investigate the effects of scaffolds, BMSCs, SDF-1 and bFGF on periodontal ligament regeneration. X-ray images and micro computed tomography (micro CT) were used to assess morphological changes in replanted teeth and surrounding alveolar bone. H&E staining and Masson's staining were also performed. BMSCs from Beagle dogs growth on scaffolds consisted of dense structured collagens. SDF-1 and bFGF effectively promoted the differentiation of BMSCs into fibroblasts, periodontal membrane reconstruction, and cell proliferation in vitro. SDF-1 and bFGF also stimulated the expression of type I collagen (Col I), type III collagen (Col III), CXC family chemokine receptor 4 (CXCR4), and S100 calcium binding protein A4 (S100A4), and decreased the expression of alkaline phosphatase (ALP). In our experimental Beagle dog model of tooth extraction and replantation, application of SDF-1 and bFGF significantly elevated periodontal membrane reconstruction and thus supported the survival of replanted teeth. In conclusion, the findings from the present study demonstrated that SDF-1 and bFGF enhance the process of periodontal ligament reconstruction, and provide a basis and reference for the use of stem cell tissue engineering in promoting periodontal membrane regeneration.
基质细胞衍生因子-1（SDF-1）和碱性成纤维细胞生长因子（bFGF）据报道可诱导骨髓间充质干细胞（BMSCs）分化为具有牙周膜成纤维细胞特征的细胞。因此，SDF-1 和 bFGF 可能在 BMSCs 介导的牙周膜再生中发挥积极作用。本研究采用噻唑蓝（MTT）比色法探讨支架、SDF-1 和 bFGF 对 BMSCs 增殖的影响。采用 RT-PCR 和 Western blot 检测基因和蛋白表达。建立犬牙再植入动物模型，探讨支架、BMSCs、SDF-1 和 bFGF 对牙周膜再生的影响。采用 X 射线和微计算机断层扫描（micro CT）评估再植牙及其周围牙槽骨的形态变化。同时进行 H&E 染色和 Masson 染色。Beagle 犬 BMSCs 在支架上生长，由密集的胶原结构组成。SDF-1 和 bFGF 可有效促进 BMSCs 向成纤维细胞分化、牙周膜重建和细胞增殖。SDF-1 和 bFGF 还可刺激 I 型胶原（Col I）、III 型胶原（Col III）、CXC 家族趋化因子受体 4（CXCR4）和 S100 钙结合蛋白 A4（S100A4）的表达，降低碱性磷酸酶（ALP）的表达。在我们的 Beagle 犬拔牙和再植动物实验模型中，应用 SDF-1 和 bFGF 可显著促进牙周膜重建，从而支持再植牙的存活。综上所述，本研究结果表明 SDF-1 和 bFGF 可增强牙周膜重建过程，为干细胞组织工程在促进牙周膜再生中的应用提供了依据和参考。
