Structure et Fonction des Membranes Biologiques, Université Libre de Bruxelles (ULB), Boulevard du Triomphe CP 206/2, B-1050 Bruxelles, Belgium.
Laboratory of Functional Neurophysiology and Pathology, Research Unit, UR/11ES09, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, 1068 Tunis, Tunisia.
Int J Mol Sci. 2021 Mar 16;22(6):3034. doi: 10.3390/ijms22063034.
The voltage-dependent anion channel (VDAC) is the major pathway for metabolites and ions transport through the mitochondrial outer membrane. It can regulate the flow of solutes by switching to a low conductance state correlated with a selectivity reversal, or by a selectivity inversion of its open state. The later one was observed in non-plant VDACs and is poorly characterized. We aim at investigating the selectivity inversion of the open state using plant VDAC purified from (PcVDAC) to evaluate its physiological role. Our main findings are: (1) The VDAC selectivity inversion of the open state occurs in PcVDAC, (2) Ion concentration and stigmasterol affect the occurrence of the open state selectivity inversion and stigmasterol appears to interact directly with PcVDAC. Interestingly, electrophysiological data concerning the selectivity inversion of the PcVDAC open state suggests that the phenomenon probably does not have a significant physiological effect in vivo.
电压依赖性阴离子通道（VDAC）是代谢物和离子通过线粒体外膜的主要途径。它可以通过切换到与选择性反转相关的低电导状态来调节溶质的流动，或者通过其开放状态的选择性反转来调节溶质的流动。后者在非植物 VDAC 中观察到，但特征描述较差。我们的目的是使用从 （PcVDAC）中纯化的植物 VDAC 研究开放状态的选择性反转，以评估其生理作用。我们的主要发现是：（1）开放状态的选择性反转发生在 PcVDAC 中，（2）离子浓度和豆甾醇影响开放状态选择性反转的发生，豆甾醇似乎直接与 PcVDAC 相互作用。有趣的是，有关 PcVDAC 开放状态选择性反转的电生理数据表明，该现象在体内可能没有显著的生理效应。
