Liao Yifei, Lupiani Blanca, Reddy Sanjay M
Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
Microorganisms. 2021 Mar 26;9(4):685. doi: 10.3390/microorganisms9040685.
Promyelocytic leukemia protein nuclear bodies (PML-NBs) are dynamic nuclear structures, shown to be important for herpesvirus replication; however, their role in regulating Marek's disease virus (MDV) infection has not been studied. MDV is an oncogenic alphaherpesvirus that causes lymphoproliferative disease in chickens. MDV encodes a US3 serine/threonine protein kinase that is important for MDV replication and gene expression. In this study, we studied the role of MDV US3 in regulating PML-NBs. Using an immunofluorescence assay, we found that MDV US3 disrupts PML and SP100 in a kinase dependent manner. In addition, treatment with MG-132 (a proteasome inhibitor) could partially restore the levels of PML and SP100, suggesting that a cellular proteasome dependent degradation pathway is involved in MDV US3 induced disruption of PML and SP100. These findings provide the first evidence for the interplay between MDV proteins and PML-NBs.
早幼粒细胞白血病蛋白核体（PML-NBs）是动态的核结构，已证明对疱疹病毒复制很重要；然而，它们在调节马立克氏病病毒（MDV）感染中的作用尚未得到研究。MDV是一种致癌的α疱疹病毒，可导致鸡的淋巴增殖性疾病。MDV编码一种US3丝氨酸/苏氨酸蛋白激酶，对MDV复制和基因表达很重要。在本研究中，我们研究了MDV US3在调节PML-NBs中的作用。使用免疫荧光测定法，我们发现MDV US3以激酶依赖性方式破坏PML和SP100。此外，用MG-132（一种蛋白酶体抑制剂）处理可部分恢复PML和SP100的水平，表明细胞蛋白酶体依赖性降解途径参与了MDV US3诱导的PML和SP100破坏。这些发现为MDV蛋白与PML-NBs之间的相互作用提供了首个证据。
