Laboratory of Parasitology, University of Manouba, National School of Veterinary Medicine of Sidi Thabet, 2020, Sidi Thabet, Tunisia; Faculty of Sciences of Tunis, University of Tunis El Manar, 2092, Manar II Tunis, Tunisia.
Laboratory of Parasitology, University of Manouba, National School of Veterinary Medicine of Sidi Thabet, 2020, Sidi Thabet, Tunisia; Faculty of Sciences of Tunis, University of Tunis El Manar, 2092, Manar II Tunis, Tunisia.
Exp Parasitol. 2021 Jun;225:108105. doi: 10.1016/j.exppara.2021.108105. Epub 2021 Apr 1.
Following the previous findings reported by the present authors on the anthelmintic effect of hydro-ethanolic extract of Mentha pulegium, the volatile constituents of M. pulegium are now assessed in the present study by exploring its anthelmintic and its antioxidant proprieties using in vitro and in vivo assays. Egg hatch assay (EHA) and adult worm's motility assays (AWMA) were used to assess the in vitro activity against Haemonchus. contortus. The in vivo anthelmintic potential was evaluated in mice infected with Heligmosomoides polygyrus using faecal egg count reduction (FECR) and total worm count reduction (TWCR). M. pulegium EO demonstrated 100% inhibition in the EHA at 200 μg/mL (IC50 = 56.36 μg/mL). In the AWM assay, EO achieved total worms paralysis 6 h after treatment exposure. This nematicidal effect was associated to morphological damages observed in the cuticular's worm using environmental scanning electron microscopy (ESEM). At 400 mg/kg, M. pulegium oil showed 75.66% of FECR and 80.23% of TWCR. The antioxidant potential of this plant was also monitored by several in vitro assays: total antioxidant capacity was 205.22 mg GAE/g DW, DPPH quenching effect was IC50 = 140 μg/mL, ABTS activity IC50 = 155 μg/mL and FRAP effect of 660 μg/mL. Regarding the in vivo assay, M. pulegium EO demonstrated a protective effect against oxidative stress by increasing the activity of the endogenous antioxidants (SOD, CAT and GPx) during H. polygyrus infection.
继本作者先前报道的唇形科薄荷属水-乙醇提取物的驱虫作用之后，本研究通过探索其驱虫作用及其抗氧化特性，评估了薄荷属植物的挥发性成分。卵孵化试验 (EHA) 和成虫运动试验 (AWMA) 用于评估其对捻转血矛线虫的体外活性。采用粪便卵计数减少 (FECR) 和总虫数减少 (TWCR) 评估其在感染旋毛虫感染的小鼠中的体内驱虫潜力。薄荷属植物精油在 200μg/mL 时对 EHA 显示出 100%的抑制作用（IC50=56.36μg/mL）。在 AWM 试验中，精油在治疗暴露后 6 小时实现了对所有蠕虫的麻痹。这种杀线虫作用与使用环境扫描电子显微镜 (ESEM) 观察到的虫体表皮的形态损伤有关。在 400mg/kg 时，薄荷属植物精油显示出 75.66%的 FECR 和 80.23%的 TWCR。该植物的抗氧化潜力也通过几种体外试验进行了监测：总抗氧化能力为 205.22mg GAE/g DW，DPPH 猝灭效应的 IC50 为 140μg/mL，ABTS 活性的 IC50 为 155μg/mL，FRAP 效应为 660μg/mL。关于体内试验，薄荷属植物精油在旋毛虫感染期间通过增加内源性抗氧化剂（SOD、CAT 和 GPx）的活性，显示出对氧化应激的保护作用。
