Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, 650500, China.
College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, China.
Mikrochim Acta. 2021 Apr 4;188(5):151. doi: 10.1007/s00604-021-04797-y.
A glucose (Glu) sensor was designed by introducing synthetic cyclic peptides (CPs) as recognition receptors and Au nanoparticles assisted graphitic carbon nitride (AuNPs/g-CN) for electrochemiluminescence (ECL) enhancement. The synthetic CP receptor (cyclo-[-CNDNHCRDNDC-]) with natural active center of Glu binding protein can mimic the interactions between Glu and Glu binding protein to specifically capture Glu. The AuNPs were reduced on g-CN and formed a new nanohybrid that can be applied as an ECL emitter. The AuNPs/g-CN effectively ameliorated the ECL response of bare g-CN. The ECL enhancement mechanism was theoretically speculated through computer simulation. Glu quantification was conducted by recording ECL shifts induced by the binding of Glu to CPs. The linear detection range of the fabricated CPs-based ECL sensor was 1 to 100 mmol L, and the detection limit (LOD) was 0.57 nmol L (S / N = 3). The CP-based ECL sensor also showed good specificity, repeatability, stability, and favorable recoveries in sample analysis. This work offer a promising analytical method for Glu assay in clinical diagnostics and bioprocess monitoring.
设计了一种葡萄糖（Glu）传感器，通过引入合成环肽（CPs）作为识别受体和金纳米粒子辅助石墨相氮化碳（AuNPs/g-CN）来增强电化学发光（ECL）。具有天然 Glu 结合蛋白结合活性中心的合成 CP 受体（cyclo-[-CNDNHCRDNDC-]）可以模拟 Glu 与 Glu 结合蛋白之间的相互作用，从而特异性捕获 Glu。AuNPs 被还原在 g-CN 上，并形成了一种新的纳米杂化材料，可用作 ECL 发射器。AuNPs/g-CN 有效地改善了裸 g-CN 的 ECL 响应。通过计算机模拟理论推测了 ECL 增强机制。通过记录 Glu 与 CPs 结合引起的 ECL 位移来进行 Glu 的定量检测。所制备的基于 CPs 的 ECL 传感器的线性检测范围为 1 至 100 mmol L，检测限（LOD）为 0.57 nmol L（S / N = 3）。基于 CP 的 ECL 传感器在样品分析中还表现出良好的特异性、重现性、稳定性和良好的回收率。这项工作为临床诊断和生物过程监测中的 Glu 分析提供了一种很有前途的分析方法。
