Bacteria Co-colonizing with in Two Asymptomatic Patients.

BACKGROUND

infection (CDI) is the leading cause of nosocomial diarrhea. Co-colonization of key bacterial taxa may prevent the transition from asymptomatic colonization to CDI. However, little is known about the composition of key bacterial taxa in asymptomatic patients.

METHODS

In the present study, the culture method was used to examine the composition of stool microbiota in two asymptomatic patients from Guizhou, China.

RESULTS

A total of 111 strains were isolated and phylogenetic relationships were determined by 16S ribosomal gene sequencing and Molecular Evolutionary Genetics Analysis version 7. The results demonstrated that (33.3%, 37/111), (24.3%, 27/111) and (11.7%, 13/111) exhibited a high ratio in asymptomatic patients. These isolates derived from two phyla: (51.3%, 57/111) and (44.1%, 49/111). In addition, co-colonization of human pathogens , , , and with was identified. To the best of our knowledge, these pathogens have not been co-isolated with previously.

CONCLUSIONS

In summary, the present study identified the composition of fecal microbiota in two asymptomatic patients in Guizhou, China. These results suggested that co-infection with human pathogens may be ubiquitous during CDI progression.