Hong Wei, Yang Jing, Cheng Yumei, Huang Xiaolin, Rao Fengqin, Zhang Ting, Wang Pixiang, Liao Jian, Qi Xiaolan, Guan Zhizhong, Chen Zhenhong, Cui Guzhen
Key Laboratory of Endemic and Ethnic Diseases, Guizhou Medical University, Ministry of Education, Guiyang 55004, Guizhou, China.
Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Guiyang 55004, Guizhou, China.
Open Life Sci. 2019 Dec 31;14:628-637. doi: 10.1515/biol-2019-0071. eCollection 2019 Jan.
infection (CDI) is the leading cause of nosocomial diarrhea. Co-colonization of key bacterial taxa may prevent the transition from asymptomatic colonization to CDI. However, little is known about the composition of key bacterial taxa in asymptomatic patients.
In the present study, the culture method was used to examine the composition of stool microbiota in two asymptomatic patients from Guizhou, China.
A total of 111 strains were isolated and phylogenetic relationships were determined by 16S ribosomal gene sequencing and Molecular Evolutionary Genetics Analysis version 7. The results demonstrated that (33.3%, 37/111), (24.3%, 27/111) and (11.7%, 13/111) exhibited a high ratio in asymptomatic patients. These isolates derived from two phyla: (51.3%, 57/111) and (44.1%, 49/111). In addition, co-colonization of human pathogens , , , and with was identified. To the best of our knowledge, these pathogens have not been co-isolated with previously.
In summary, the present study identified the composition of fecal microbiota in two asymptomatic patients in Guizhou, China. These results suggested that co-infection with human pathogens may be ubiquitous during CDI progression.
艰难梭菌感染（CDI）是医院获得性腹泻的主要原因。关键细菌类群的共同定殖可能会阻止从无症状定殖向CDI的转变。然而，对于无症状患者中关键细菌类群的组成知之甚少。
在本研究中，采用培养方法检测了两名来自中国贵州的无症状患者粪便微生物群的组成。
共分离出111株菌株，并通过16S核糖体基因测序和分子进化遗传学分析第7版确定了系统发育关系。结果表明，[具体细菌名称1]（33.3%，37/111）、[具体细菌名称2]（24.3%，27/111）和[具体细菌名称3]（11.7%，13/111）在无症状患者中占比很高。这些分离株来自两个门：[具体门名称1]（51.3%，57/111）和[具体门名称2]（44.1%，49/111）。此外，还鉴定出人类病原体[具体病原体名称1]、[具体病原体名称2]、[具体病原体名称3]、[具体病原体名称4]和[具体病原体名称5]与[某种细菌]的共同定殖。据我们所知，这些病原体以前尚未与[某种细菌]共同分离出来。
总之，本研究确定了中国贵州两名无症状患者粪便微生物群的组成。这些结果表明，在CDI进展过程中，与人类病原体的共同感染可能很普遍。
