高血压性视网膜病变患者的谷胱甘肽相关抗氧化防御系统。

Glutathione-related antioxidant defense system in patients with hypertensive retinopathy.

机构信息

Department of Biochemistry and Clinical Biochemistry, "Nicolae Testemițanu" State University of Medicine and Pharmacy, Chișinău, Republic of Moldova.

Laboratory of Biochemistry, "Nicolae Testemițanu" State University of Medicine and Pharmacy, Chișinău, Republic of Moldova.

出版信息

Rom J Ophthalmol. 2021 Jan-Mar;65(1):46-53. doi: 10.22336/rjo.2021.9.

DOI:10.22336/rjo.2021.9

PMID:33817433

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7995505/

Abstract

To analyze the glutathione antioxidant defense system changes in the tear and serum of patients with hypertensive retinopathy (HR) and to establish whether there is an interdependence between their levels and HR degree. 90 patients were split into three groups according to the Keith-Wagner-Barker grading of HR: GI-36 patients; GII-35 patients; GIII-19 patients. The concentration of reduced glutathione (GSH) and activities of glutathione peroxidase (GPx) and glutathione reductase (GR) in tear and serum were measured. Results were analyzed by ANOVA, followed by Bonferroni test. The Spearman correlation coefficient was calculated (p≤0.05 statistically significant). In serum, the GSH level and GPx activity were not statistically changed between groups with HR degree advancement, unlike the GR activity that was statistically diminished (p=0.018). The values of the studied markers in the tear showed a decrease with the progression of the HR degree. Only serum GSH level correlated with the tear one (r=-0.361, p=0.000), while the enzymes activity did not. A correlation of GPx and GR activity (r=0.417, p=0.000) was identified in tear, while in serum - of GPx activity and GSH level (r=409, p=0.000). Tear GPx and GR levels correlated significantly but with low power with HR degree (r=0.299, p=0.004/ r=0.299, p=0.004). Statistically significant elevation in tear GPx and GR activity and a tendency of GSH level increase was revealed, being attested, and a direct correlation between GPx and GR activity, as well as of their activity with the HR degree. In serum, the GSH level and the GPx activity did not change accurately, while the GR activity diminished significantly, the identified decrease being correlated with the HR degree. HR = hypertensive retinopathy, HTN = hypertension, GSH = reduced glutathione, GPx = glutathione peroxidase, GR = glutathione reductase, GGR = gamma-glutamyl transferase, ROSs = reactive species of oxygen, OS = oxidative stress.

摘要

分析高血压性视网膜病变（HR）患者的泪液和血清中谷胱甘肽抗氧化防御系统的变化，并确定其水平与 HR 程度之间是否存在相互依存关系。根据 Keith-Wagner-Barker 分级法，将 90 例患者分为三组：GI-36 例患者；GII-35 例患者；GIII-19 例患者。测量泪液和血清中还原型谷胱甘肽（GSH）的浓度和谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）的活性。采用方差分析对结果进行分析，然后进行 Bonferroni 检验。计算 Spearman 相关系数（p≤0.05 有统计学意义）。血清中，随着 HR 程度的进展，GSH 水平和 GPx 活性没有统计学差异，而 GR 活性则明显降低（p=0.018）。泪液中研究标志物的数值随 HR 程度的进展而降低。只有血清 GSH 水平与泪液中的 GSH 水平相关（r=-0.361，p=0.000），而酶活性则没有相关性。在泪液中发现 GPx 和 GR 活性之间存在相关性（r=0.417，p=0.000），而在血清中则是 GPx 活性与 GSH 水平之间存在相关性（r=409，p=0.000）。泪液中 GPx 和 GR 水平与 HR 程度显著相关，但相关性较低（r=0.299，p=0.004/ r=0.299，p=0.004）。结果表明，泪液中 GPx 和 GR 活性升高，GSH 水平有升高趋势，GPx 和 GR 活性之间以及它们的活性与 HR 程度之间存在直接相关性。在血清中，GSH 水平和 GPx 活性没有准确变化，而 GR 活性明显降低，发现这种降低与 HR 程度相关。HR=高血压性视网膜病变，HTN=高血压，GSH=还原型谷胱甘肽，GPx=谷胱甘肽过氧化物酶，GR=谷胱甘肽还原酶，GGR=γ-谷氨酰转移酶，ROSs=活性氧，OS=氧化应激。