General Incorporated Association the Institute for Hydrogen Medicine, Kobe, Japan.
General Incorporated Association the Institute for Hydrogen Medicine, Kobe; Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Shobara, Japan.
Med Gas Res. 2021 Apr-Jun;11(2):61-65. doi: 10.4103/2045-9912.311496.
Hydrogen molecules have attracted attention as a new antioxidant, but are left to be confirmedly verified whether the oral administration is highly safe or not, concurrently with retention of abundant hydrogen. When electrolysis was performed for 10 minutes using a direct-current electrolytic hydrogen-water generating bottle with tap water, "residual free chlorine" concurrently upon the production of molecular hydrogen (444 μg/L) could be appreciably decreased from 0.18 mg/L to 0.12 mg/L as quantified by a N,N-diethyl-p-phenylenediamine-dye colorimetric method. Moreover, the total chlorine concentration (residual bound chlorine plus free chlorine) was estimated to be decreased from 0.17 mg/L to 0.11 mg/L. Although a merit of electrolytic hydrogen-generating bottles exists in electrolysis for periods as short as 10 minutes, the 30-minute electrolysis brought about the more abundant hydrogen (479 μg/L) together with an oxidation-reduction potential of -245 mV; even upon this long-term electrolysis, the gross amounts of chlorine, hypochlorous acid and chloramine were shown not to be increased (0.09-0.10 mg/L from 0.11 mg/L for tap water) as detected by orthotolidine colorimetry. Above-mentioned levels of diverse-type chlorines might fulfill the World Health Organization guideline for drinking water below 5 mg/L. In addition, the dissolved ozone upon electrolytic generation of hydrogen-water was below the detection limit (< 0.05 mg/L) or undetectable, which fulfilled the official safety standards in Japan and the USA for drinking water below 0.1 mg/L, as evaluated by three methods such as an electrode-type ozone checker, indigo dye-utilizing ozone detector capillaries and potassium iodide-based colorimetry. Importantly, even when half the amount of tap water was poured into the tank of the apparatus and electrolyzed, both the residual chlorine and ozone concentrations measured were also below the safety standard. Thus, major potently harmful substances, such as residual free/bound chlorine, or hypochlorous-acid/chloramine, respectively, and dissolved ozone, as the drinking hydrogen-water was direct-current-electrolytically generated, were estimated to be repressed within safety concentration ranges with achievements of abundant hydrogen generation.
氢气分子作为一种新型抗氧化剂备受关注，但口服给药的高度安全性仍有待证实，同时要保持大量氢气的存在。使用自来水进行 10 分钟的直流电电解氢气水生成瓶电解时，通过 N,N-二乙基对苯二胺比色法定量，可将分子氢（444μg/L）生成时同时产生的“游离余氯”（444μg/L）从 0.18mg/L 显著降低至 0.12mg/L。此外，总氯浓度（结合氯加游离氯）估计从 0.17mg/L 降低至 0.11mg/L。虽然电解氢气生成瓶的优点在于可以在 10 分钟的短时间内进行电解，但 30 分钟的电解产生了更丰富的氢气（479μg/L），同时氧化还原电位为-245mV；即使进行这种长期电解，总氯、次氯酸和氯胺的总量也没有增加（从自来水的 0.11mg/L 增加到 0.09-0.10mg/L），这是通过邻联甲苯胺比色法检测到的。上述各种类型的氯含量可能符合世界卫生组织饮用水低于 5mg/L 的指导方针。此外，通过电解氢气水生成的溶解臭氧低于检测限（<0.05mg/L）或无法检测到，这通过三种方法（电极型臭氧检测仪、利用靛蓝染料的臭氧检测帽毛细管和基于碘化钾的比色法）评估，符合日本和美国饮用水低于 0.1mg/L 的官方安全标准。重要的是，即使将一半量的自来水倒入仪器的水箱并进行电解，测量的余氯和臭氧浓度也低于安全标准。因此，在直接电流电解产生饮用氢气水时，估计主要的潜在有害物质，如游离/结合氯、次氯酸/氯胺，以及溶解臭氧，都被抑制在安全浓度范围内，同时实现了大量氢气的生成。
