General Courses Unit, Faculty of Sciences and Arts, King Khalid University, Dhahran Aljanoub, Saudi Arabia.
Luminescence. 2021 Aug;36(5):1231-1238. doi: 10.1002/bio.4048. Epub 2021 Apr 14.
α-Difluoromethylornithine is an effective medication for the treatment of African Trypanosomiasis and widely distributed for the treatment of hirsutism. This work provides an adequate analytical protocol for the spectrophotometric and the spectrofluorimetric determination of α-difluoromethylornithine through its interaction with 4-chloro-7-nitrobenzofurazan (NBD-chloride) reagent. After optimization of the reaction conditions (NBD-chloride volume, buffer volume, the best diluting solvent, heating time and temperature and pH of the medium) the reaction product was measured spectrophotometrically at λ = 478 nm and spectrofluorimetrically at λ = 540 nm after λ = 475 nm. The proposed methods were linear over the ranges 5-30 μg ml and 0.4-2 μg ml for the spectrophotometric method and the spectrofluorimetric method, respectively. Moreover, the proposed work offers an adequate sensitive and selective determination for α-difluoromethylornithine where the detection limits were 0.90 μg ml and 0.071 μg ml for the spectrophotometric method and the spectrofluorimetric method, respectively. Furthermore, both methods were successfully applied for the quantification of α-difluoromethylornithine in the pharmaceutical cream samples with acceptable recovery results.
α-二氟甲基鸟氨酸是一种有效的抗非洲锥虫病药物，广泛用于治疗多毛症。本工作通过其与 4-氯-7-硝基苯并呋咱（NBD-氯）试剂的相互作用，提供了一种用于分光光度法和荧光光度法测定α-二氟甲基鸟氨酸的充分分析方案。在优化反应条件（NBD-氯体积、缓冲液体积、最佳稀释溶剂、加热时间和温度以及介质的 pH 值）后，在 λ = 478 nm 处分光光度法和 λ = 540 nm 处荧光光度法后测量反应产物，λ = 475 nm。该分光光度法和荧光光度法的线性范围分别为 5-30 μg/ml 和 0.4-2 μg/ml。此外，该方法提供了一种灵敏且选择性好的α-二氟甲基鸟氨酸测定方法，其检测限分别为 0.90 μg/ml 和 0.071 μg/ml。此外，这两种方法都成功地应用于药物霜剂样品中α-二氟甲基鸟氨酸的定量分析，具有可接受的回收率结果。
