Almahri Albandary, Abdel-Lateef Mohamed A
General Courses Unit, Faculty of Sciences and Arts, King Khalid University, Dhahran Aljanoub, Saudi Arabia.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt.
R Soc Open Sci. 2021 May 12;8(5):210366. doi: 10.1098/rsos.210366.
Eflornithine (EFN) is an anti- agent for the medication of sleeping sickness and widely distributed for the treatment of hirsutism (unwanted facial hair in women). The presented work demonstrates a comprehensive analytical approach for the spectrofluorometric determination of EFN in commercial cream samples and various biological samples. The proposed method is based on the formation of a highly yellow-green fluorescence dihydropyridine derivative after the interaction between EFN and acetylacetone/formaldehyde reagent in a slightly acidic medium. Furthermore, the optimal variables such as reagent volumes, pH of the medium, heating time, buffer volume, heating temperature and diluting solvent were carefully selected to achieve the maximum fluorescence activity. The fluorescence activity for the formed derivative was measured at = 477 nm after = 418 nm. Concerning linearity, accuracy, sensitivity, precision and robustness, the presented method was validated and verified according to ICH guidelines. Moreover, the proposed work offered a selective determination for EFN in various brands of pharmaceutical cream without any interference from excipients. Eventually, the current approach was assured to be successful in the estimation of EFN in urine and plasma samples with acceptable recovery results.
依氟鸟氨酸（EFN）是一种用于治疗昏睡病的抗寄生虫药物，广泛用于治疗多毛症（女性面部多余毛发）。本文展示了一种用于分光荧光法测定市售乳膏样品和各种生物样品中依氟鸟氨酸的综合分析方法。所提出的方法基于在微酸性介质中依氟鸟氨酸与乙酰丙酮/甲醛试剂相互作用后形成高黄绿色荧光二氢吡啶衍生物。此外，仔细选择了试剂体积、介质pH值、加热时间、缓冲液体积、加热温度和稀释溶剂等最佳变量，以实现最大荧光活性。在激发波长为418nm后，在发射波长为477nm处测量形成衍生物的荧光活性。关于线性、准确性、灵敏度、精密度和稳健性，根据国际人用药品注册技术协调会（ICH）指南对所提出的方法进行了验证和确认。此外，所提出的工作为各种品牌的药用乳膏中的依氟鸟氨酸提供了选择性测定方法，不受辅料干扰。最终，目前的方法在尿液和血浆样品中依氟鸟氨酸的测定中取得成功，回收率结果可接受。
