Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Research Center for Traditional Medicine and History of Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Arch Razi Inst. 2021 Mar;76(1):95-105. doi: 10.22092/ari.2019.126702.1356. Epub 2021 Mar 1.
Chemotherapy is the main approach for the treatment of cancer; however, it often causes unpleasant oxidative damages. Therefore, the development of an effective alternative/complementary therapy with improved tumor suppression efficiency and lower adverse effects is highly required. Recently, it has been shown that Cyrtopodion scabrum extract (CsE) is an effective and selective tumor suppressor medicine. The present study investigated the antioxidant activity of Cyrtopodion scabrum homogenate (CsH) and CsE and their effects on attenuating 5-fluorouracil (5-FU)-induced liver dysfunction in rats. A total of 60 male rats (weight: 200-220 g) were divided into six groups and treated for 14 days. The control (group I) and 5-FU (group II) groups received distilled water and 5-FU, respectively. The other four groups were orally administered with CsE, CsH, CsE+5-FU, and CsH+5-FU (groups III to VI), respectively by gavages based on a daily schedule. The 5-FU-induced oxidative damage was evaluated by changes in the weight and food and water intake during the treatment and antioxidant parameters in the liver and serum of the treated rats. The obtained data indicated that the administration of CsH and CsE significantly improved liver function and defense system of antioxidants by attenuating the levels or activities of malondialdehyde, superoxide anion, aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase and decrease of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase, total antioxidant capacity, glutathione, total protein, and albumin in the liver and serum, induced by 5-FU treatment. The obtained data of the current study suggested that CsH and CsE play a protective role in the imbalance elicited by 5-FU and can be used as alternative/complementary supplements with 5-FU to reduce oxidative damages which is the consequence of reactive oxygen species production in cancerous patients.
化疗是治疗癌症的主要方法；然而，它常常会引起不愉快的氧化损伤。因此，开发一种有效且安全的替代/补充疗法，提高肿瘤抑制效率，降低不良反应，是非常必要的。最近，已经表明，草乌头提取物（CsE）是一种有效的、选择性的肿瘤抑制剂。本研究旨在探讨 Cyrtopodion scabrum 匀浆（CsH）和 CsE 的抗氧化活性及其对减轻氟尿嘧啶（5-FU）诱导的大鼠肝损伤的作用。共 60 只雄性大鼠（体重：200-220g）分为 6 组，连续处理 14 天。对照组（I 组）和 5-FU 组（II 组）分别给予蒸馏水和 5-FU。其余四组大鼠分别经灌胃给予 CsE、CsH、CsE+5-FU 和 CsH+5-FU（III 组至 VI 组），每日一次。通过观察治疗过程中体重、食物和水的摄入变化以及处理后大鼠肝和血清中的抗氧化参数，评估 5-FU 诱导的氧化损伤。结果表明，CsH 和 CsE 可通过降低肝和血清中丙二醛、超氧阴离子、天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶的水平或活性，以及超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽 S-转移酶、总抗氧化能力、谷胱甘肽、总蛋白和白蛋白的活性，改善肝脏功能和抗氧化防御系统，减轻 5-FU 诱导的氧化损伤。本研究结果表明，CsH 和 CsE 对 5-FU 诱导的失衡具有保护作用，可与 5-FU 联合使用作为替代/补充疗法，减少癌症患者活性氧产生导致的氧化损伤。
