Gohari Ali, Noorafshan Ali, Akmali Masoumeh, Zamani-Garmsiri Fahimeh, Seghatoleslam Atefeh
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Med Sci. 2018 Mar;43(2):174-183.
is known as an anti-hyperglycemic plant. distillate (UD) is a traditional Iranian drink, locally known as "aragh gazaneh". In spite of its widespread consumption in Iran, according to traditional Iranian medicine, there is no scientific report on the usefulness of UD for diabetic patients. This survey was designed to evaluate its protective effects for the recovery from diabetes by determining the serum insulin, blood glucose, volume of pancreatic islets, and the number and volume of β-cells in diabetic rats.
A total of 48 Sprague-Dawley male rats (200-250 g) were randomly distributed into 6 groups (n=8), including non-diabetic plus distilled water (DW), non-diabetic plus UD, diabetic plus DW, diabetic plus UD, diabetic plus insulin, and diabetic plus glibenclamide. DW, UD, and glibenclamide were administered via intragastric gavage and insulin was injected subcutaneously. After four weeks of experiments, blood samples were collected for serum insulin and blood glucose assay. Pancreas was also evaluated using stereological method. The SPSS software was used for statistical analysis. Kruskal-Wallis, repeated measurements, and Mann-Whitney U test were applied for comparisons between the groups.
The treatment of diabetic rats with UD reduced the blood glucose dramatically (P<0.001) and increased serum insulin levels significantly (P=0.03) in comparison to the diabetic plus DW rats. Treatment with UD did not affect the mean β-cell volumes in the diabetic rats when compared to the diabetic plus DW rats, but the islet volumes and β-cell numbers were significantly recovered.
UD treatment in diabetic rats improves hyperglycemia by partially restoring plasma insulin levels. The data suggest that UD prevents islet atrophy and/or regenerate pancreatic β-cells.
[植物名称]被认为是一种抗高血糖植物。[植物名称]馏出物（UD）是一种传统的伊朗饮品，当地称为“aragh gazaneh”。尽管它在伊朗广泛消费，但根据传统的伊朗医学，尚无关于UD对糖尿病患者有用性的科学报告。本研究旨在通过测定糖尿病大鼠的血清胰岛素、血糖、胰岛体积以及β细胞的数量和体积，评估其对糖尿病恢复的保护作用。
总共48只Sprague-Dawley雄性大鼠（200 - 250克）被随机分为6组（n = 8），包括非糖尿病加蒸馏水（DW）组、非糖尿病加UD组、糖尿病加DW组、糖尿病加UD组、糖尿病加胰岛素组和糖尿病加格列本脲组。DW、UD和格列本脲通过灌胃给药，胰岛素皮下注射。实验四周后，采集血样进行血清胰岛素和血糖测定。胰腺也采用体视学方法进行评估。使用SPSS软件进行统计分析。Kruskal-Wallis检验、重复测量和Mann-Whitney U检验用于组间比较。
与糖尿病加DW组大鼠相比，用UD治疗糖尿病大鼠可显著降低血糖（P < 0.001）并显著提高血清胰岛素水平（P = 0.03）。与糖尿病加DW组大鼠相比，用UD治疗对糖尿病大鼠的平均β细胞体积没有影响，但胰岛体积和β细胞数量显著恢复。
糖尿病大鼠接受UD治疗可通过部分恢复血浆胰岛素水平改善高血糖。数据表明，UD可防止胰岛萎缩和/或使胰腺β细胞再生。
