National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan; Department of Parasitology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan; Laboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.
Infect Genet Evol. 2021 Aug;92:104838. doi: 10.1016/j.meegid.2021.104838. Epub 2021 Apr 2.
Neospora caninum is one of the main causes of bovine abortions worldwide, including Japan. Nothing is known about the N. caninum population substructures in Japan, and only one isolate from a pregnant sheep has been studied to date. This study describes, for the first time, the genetic characterization of isolates of N. caninum implicated in cattle abortions in Japan. Brains from five aborted fetuses were successfully genotyped based on multilocus microsatellite markers. Assigned genotypes showed high frequencies of mixed alleles in the sequenced markers MS7 and MS10, raising concerns about the subpopulation structures of N. caninum infecting animals in Japan. Clustering analysis of the genotypes, together with those from a previous dataset, showed that five of the six genotypes were distinct from other clusters. Meanwhile, the remaining genotype, together with the sheep isolate from Japan, was grouped with those from Mexico and Spain. These preliminary data may indicate a complex transmission pattern of N. caninum in Japan via clonal spreading by vertical and horizontal transmission and geographically related population substructuring.
刚地弓形虫是世界范围内引起牛流产的主要原因之一，包括日本。目前尚不清楚日本刚地弓形虫种群的亚结构，迄今为止，仅对来自一只怀孕绵羊的一个分离株进行了研究。本研究首次描述了与日本牛流产有关的刚地弓形虫分离株的遗传特征。基于多位点微卫星标记，成功对来自 5 个流产胎儿的大脑进行了基因分型。分配的基因型在测序标记 MS7 和 MS10 中显示出混合等位基因的高频率，这引起了对日本感染动物的刚地弓形虫亚种群结构的关注。基因型的聚类分析，以及之前数据集的分析结果表明，6 种基因型中的 5 种与其他聚类不同。同时，其余的基因型与来自日本的绵羊分离株一起与来自墨西哥和西班牙的分离株聚为一类。这些初步数据可能表明，日本的刚地弓形虫传播模式复杂，通过垂直和水平传播的克隆传播以及与地理相关的种群亚结构。
