产朊假丝酵母中的钴胺素及钴胺素依赖性酶
Cobalamins and cobalamin-dependent enzymes in Candida utilis.
作者信息
Poston J M, Hemmings B A
出版信息
J Bacteriol. 1979 Dec;140(3):1013-6. doi: 10.1128/jb.140.3.1013-1016.1979.
Abstract
Candida utilis has been shown to contain 4.7 pmol of cobalamin per g of wet cell paste. Purification of the cobalamin showed it to be a mixture of methylcobalamin and adenosylcobalamin. Two cobalamin-dependent enzyme systems have been found in the yeast: methylcobalamin-dependent methionine biosynthesis and leucine 2,3-aminomutase. The cobalamin extracted from the yeast is as effective as authentic adenosylcobalamin in stimulating leucine 2,3-aminomutase.
摘要
已表明产朊假丝酵母每克湿细胞糊中含有4.7皮摩尔的钴胺素。钴胺素的纯化表明它是甲基钴胺素和腺苷钴胺素的混合物。在酵母中发现了两种钴胺素依赖性酶系统：甲基钴胺素依赖性蛋氨酸生物合成和亮氨酸2,3-氨基变位酶。从酵母中提取的钴胺素在刺激亮氨酸2,3-氨基变位酶方面与纯腺苷钴胺素一样有效。
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