用若丹宁测定没食子单宁。
Determination of gallotannin with rhodanine.
作者信息
Inoue K H, Hagerman A E
机构信息
Department of Chemistry, Miami University, Oxford, Ohio 45056.
出版信息
Anal Biochem. 1988 Mar;169(2):363-9. doi: 10.1016/0003-2697(88)90296-5.
PMID:3382009
Abstract
A reliable method for quantitative analysis of gallotannin in plants has been devised. Gallotannin is hydrolyzed with acid, and gallic acid in the hydrolysate is then assayed using rhodanine. This method is very specific; no interferences from other plant phenolics, including ellagic acid and condensed tannin, have been observed. The rhodanine assay has a sensitivity of 0.01 mg of gallic acid and a precision of 2.2% (relative standard deviation).
摘要
已设计出一种可靠的植物中没食子单宁定量分析方法。没食子单宁用酸水解，然后用罗丹宁测定水解产物中的没食子酸。该方法具有很高的特异性；未观察到其他植物酚类物质（包括鞣花酸和缩合单宁）的干扰。罗丹宁测定法对没食子酸的灵敏度为0.01毫克，精密度为2.2%（相对标准偏差）。
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