Olennikov Daniil N, Chemposov Vladimir V, Chirikova Nadezhda K
Laboratory of Medical and Biological Research, Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, 670047 Ulan-Ude, Russia.
Department of Biology, Institute of Natural Sciences, North-Eastern Federal University, 677027 Yakutsk, Russia.
Plants (Basel). 2021 Nov 20;10(11):2525. doi: 10.3390/plants10112525.
Prickly rose ( Lindl.) is the most distributed rose species in the Northern Hemisphere, used by indigenous people for various food purposes. The lack of detailed information about the chemical composition of has led us to study the phytochemical composition and metabolic profile of prickly rose extracts using chromatographic techniques. Many groups of phenolic and non-phenolic compounds were quantified in the leaves, flowers, roots and fruits of . Phenolic compounds were the dominant phytochemicals in the aerial parts and roots of . A precise study by high-performance liquid chromatography with photodiode array detection and electrospray ionization triple quadrupole mass spectrometric detection showed the presence of 123 compounds, among which ellagic acid derivatives, ellagitannins, gallotannins, catechins, catechin oligomers, hydroxycinnamates and flavonoid glycosides of kaempferol, quercetin and dihydroquercetin were all identified for the first time. The most abundant phenolic compounds were ellagitannins and flavonoid glycosides, with a maximal content of 70.04 mg/g in leaves and 66.72 mg/g in flowers, respectively, indicating the great ability of organs to accumulate phenolic compounds. By applying a standardized static, simulated gastrointestinal digestion method, we found the inhibitory potential of the leaf extract against digestive α-amylases. A pancreatic α-amylase activity-inhibiting assay coupled with HPLC microfractionation demonstrated high inhibition of enzyme activity by ellagitannin rugosin D, which was later confirmed by a microplate reaction with mammalian α-amylases and the simulated digestion method. This study clearly demonstrates that leaf extract and its main component, ellagitannin rugosin D, strongly inhibit digestive α-amylase, and may be a prospective antidiabetic agent.
多刺蔷薇（Lindl.）是北半球分布最广的蔷薇物种，当地居民将其用于各种食物用途。由于缺乏关于其化学成分的详细信息，我们采用色谱技术对多刺蔷薇提取物的植物化学成分和代谢谱进行了研究。在多刺蔷薇的叶、花、根和果实中对多组酚类和非酚类化合物进行了定量分析。酚类化合物是多刺蔷薇地上部分和根中的主要植物化学成分。通过高效液相色谱-光电二极管阵列检测和电喷雾电离三重四极杆质谱检测进行的精确研究表明，共存在123种化合物，其中鞣花酸衍生物、鞣花单宁、没食子单宁、儿茶素、儿茶素低聚物、羟基肉桂酸以及山奈酚、槲皮素和二氢槲皮素的黄酮苷均为首次鉴定。含量最丰富的酚类化合物是鞣花单宁和黄酮苷，在叶中的最大含量分别为70.04毫克/克，在花中的最大含量为66.72毫克/克，表明多刺蔷薇各器官积累酚类化合物的能力很强。通过应用标准化的静态模拟胃肠道消化方法，我们发现叶提取物对消化性α-淀粉酶具有抑制潜力。胰α-淀粉酶活性抑制试验结合高效液相色谱微分离显示，鞣花单宁rugosin D对酶活性具有高度抑制作用，随后通过与哺乳动物α-淀粉酶的微孔板反应和模拟消化方法得到了证实。这项研究清楚地表明，多刺蔷薇叶提取物及其主要成分鞣花单宁rugosin D能强烈抑制消化性α-淀粉酶，可能是一种有前景的抗糖尿病药物。
