Pesce M A, Giacomo D F
Columbia Presbyterian Medical Center, Special Chemistry Laboratory, New York, NY 10032.
Ann Clin Lab Sci. 1988 Mar-Apr;18(2):168-73.
Blood is drawn into capillary tubes containing saponin and the tubes placed into the reagent packs. Hemoglobin is denatured by mixing the hemosylate with a reagent containing lithium hydroxide and a non-ionic detergent. The absorbance is measured bichromatically at wavelengths of 577 and 633 nm. The calibration curve is stable and can be stored for at least 30 days. There are no interferences from fetal hemoglobin, glycosylated hemoglobin (20 percent), hemoglobin S, samples with hematocrits up to 0.55, paraproteins, and lipemia. Specimens with rouleau formation, nucleated and fragmented red blood cells, target cells, ovalocytes, teardrop cells, spherocytes, leukocyte counts of 29 X 10(9) per L and reticulocyte counts of 0.32; Howell-Jolly bodies did not interfere with the assay. The within run and between run precision gave average coefficient or variations of 2.3 and 1.9 percent, respectively. Comparison of the hemoglobin results obtained in 149 samples with the Vision (y) and Coulter Counter System (x) gave r = 0.987, Y = 1.01X - 1.89 g per L.
将血液抽入含有皂角苷的毛细管中，然后将这些毛细管放入试剂包中。通过将溶血产物与含有氢氧化锂和非离子洗涤剂的试剂混合，使血红蛋白变性。在577和633nm波长处进行双色吸光度测量。校准曲线稳定，可保存至少30天。胎儿血红蛋白、糖化血红蛋白（20%）、血红蛋白S、血细胞比容高达0.55的样本、副蛋白和脂血均无干扰。有缗钱状形成、有核和破碎红细胞、靶形细胞、椭圆形红细胞、泪滴形细胞、球形红细胞、白细胞计数为29×10⁹/L和网织红细胞计数为0.32的样本；豪-焦小体不干扰测定。批内和批间精密度的平均变异系数分别为2.3%和1.9%。对149个样本中使用Vision（y）和库尔特计数器系统（x）获得的血红蛋白结果进行比较，得到r = 0.987，Y = 1.01X - 1.89 g/L。
