Wylie B R, Lovric V A
Red Cross Blood Transfusion Service, Sydney.
Pathology. 1988 Apr;20(2):152-5. doi: 10.3109/00313028809066626.
Using purified chlorohemin as a stable standard, a newly described hemoglobinometry method was evaluated. All blood samples as well as chlorohemin gave identical absorption spectra in an alkaline-detergent solution with a broad peak at 575 nm. Both the reagent mixture and the chlorohemin standard were stable for at least 250 days when stored between 8 degrees C and 32 degrees C in brown glass bottles. The new method showed close correlation when compared to the cyanmethemoglobin technique. Compared to the cyanmethemoglobin method the advantages included a stable standard, shorter conversion time, reduced plasma background and improved hemoglobin ligands conversion. We conclude that the new method confers significant advantages over the cyanmethemoglobin method and in particular provides a stable primary standard with a long shelf-life.
以纯化氯血红素作为稳定标准物，对一种新描述的血红蛋白测定方法进行了评估。所有血样以及氯血红素在碱性去污剂溶液中均呈现相同的吸收光谱，在575nm处有一个宽峰。当在棕色玻璃瓶中于8摄氏度至32摄氏度之间储存时，试剂混合物和氯血红素标准物至少250天内保持稳定。与氰化高铁血红蛋白技术相比，新方法显示出密切相关性。与氰化高铁血红蛋白方法相比，其优点包括标准物稳定、转换时间更短、血浆背景降低以及血红蛋白配体转换得到改善。我们得出结论，新方法相对于氰化高铁血红蛋白方法具有显著优势，特别是提供了一种具有长保质期的稳定一级标准物。
