使用Hemocue系统进行胎儿血红蛋白定量快速且准确。

Fetal hemoglobin quantitations using the Hemocue system are rapid and accurate.

作者信息

Berry S M, Dombrowski M P, Blessed W B, Bichalski J A, Jones T B, Cotton D B

机构信息

Department of Obstetrics and Gynecology, Wayne State University/Hutzel Hospital, Detroit, Michigan.

出版信息

Obstet Gynecol. 1993 Mar;81(3):417-20.

PMID:7679787

Abstract

OBJECTIVES

To determine whether hemoglobin quantitations using the Hemocue system, a rapid and portable hemoglobin photometer, on fetal blood obtained via funipuncture were accurate compared to the Coulter S-Plus IV. We also examined whether gestational age or extremes in hemoglobin levels significantly affected the accuracy of the Hemocue system.

METHODS

We performed fetal hemoglobin quantitations using both systems on 58 specimens obtained between 18-38 weeks' gestation. Correlation between values by both systems was determined by linear regression analysis. The effects of gestational age and hemoglobin extremes on the accuracy of the Hemocue system were evaluated by stepwise regression.

RESULTS

The mean Hemocue value was 12.0 +/- 2.4 g/dL (range 3.3-16.4); the mean Coulter value was 11.7 +/- 2.3 g/dL (range 3.6-16.2). The regression equation for Hemocue (y) versus Coulter (x) values was y = 0.72 + 0.97x; r = 0.94 (P < .0001). Neither gestational age nor hemoglobin extremes significantly affected the accuracy of the Hemocue system.

CONCLUSION

The Hemocue system is rapid and accurate for fetal hemoglobin quantitation between 18-38 weeks' gestation regardless of extremes in gestational age or hemoglobin levels.

摘要

目的

确定使用Hemocue系统（一种快速便携的血红蛋白光度计）对经脐静脉穿刺获取的胎儿血液进行血红蛋白定量检测，与使用库尔特S-Plus IV血细胞分析仪相比是否准确。我们还研究了胎龄或血红蛋白水平的极端值是否会显著影响Hemocue系统的准确性。

方法

我们使用这两种系统对58例妊娠18至38周的标本进行胎儿血红蛋白定量检测。通过线性回归分析确定两种系统检测值之间的相关性。通过逐步回归评估胎龄和血红蛋白极端值对Hemocue系统准确性的影响。

结果

Hemocue系统的平均检测值为12.0±2.4 g/dL（范围3.3 - 16.4）；库尔特血细胞分析仪的平均检测值为11.7±2.3 g/dL（范围3.6 - 16.2）。Hemocue（y）与库尔特（x）检测值的回归方程为y = 0.72 + 0.97x；r = 0.94（P <.0001）。胎龄和血红蛋白极端值均未显著影响Hemocue系统的准确性。