Yu Jia, Qin Mei, Li Juan, Cui Shumin
Department of Ophthalmology, The First Affiliated Hospital of Bengbu Medical College, Bengbu City, People's Republic of China.
Arch Physiol Biochem. 2023 Oct;129(5):1117-1122. doi: 10.1080/13813455.2021.1900873. Epub 2021 Apr 6.
This study aimed to investigate the role of long non-coding RNA (lncRNA) small nucleolar RNA host gene 4 (SNHG4) in diabetic retinopathy (DR). We found that SNHG4 was downregulated in DR. SNHG4 could directly interact with miR-200b, while overexpression of miR-200b did not affect the expression of SNHG4 in human retinal pigment epithelial cells ARPE-19. In contrast, overexpression of SNHG4 led to the upregulation of oxidation resistance 1 (Oxr1), a target of miR-200b. Cell apoptosis analysis showed that overexpression of miR-200b increased the apoptotic rate of ARPE-19 cells under high glucose treatment. Oxr1 and SNHG4 played opposite roles and reduced the effects of overexpression of miR-200b. In conclusion, SNHG4 may sponge miR-200b to inhibit cell apoptosis in DR by upregulating Oxr1.
本研究旨在探讨长链非编码RNA（lncRNA）小核仁RNA宿主基因4（SNHG4）在糖尿病视网膜病变（DR）中的作用。我们发现SNHG4在DR中表达下调。SNHG4可直接与miR-200b相互作用，而miR-200b的过表达并不影响人视网膜色素上皮细胞ARPE-19中SNHG4的表达。相反，SNHG4的过表达导致miR-200b的靶标抗氧化1（Oxr1）上调。细胞凋亡分析表明，miR-200b的过表达增加了高糖处理下ARPE-19细胞的凋亡率。Oxr1和SNHG4发挥相反作用，并减弱了miR-200b过表达的影响。总之，SNHG4可能通过上调Oxr1来吸附miR-200b，从而抑制DR中的细胞凋亡。
