Concentrating Pooled COVID-19 Patient Lysates to Improve Reverse Transcription Quantitative PCR Sensitivity and Efficiency.
作者信息
Sanghani Harshmeena R, Nawrot Dorota A, Marmolejo-Cossío Francisco, Taylor John M, Craft Judith, Kalimeris Elizabeth, Andersson Monique I, Vasudevan Sridhar R
机构信息
Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, UK.
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, UK.
出版信息
Clin Chem. 2021 Apr 29;67(5):797-798. doi: 10.1093/clinchem/hvab035.
Abstract
摘要
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本文引用的文献
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A new coronavirus associated with human respiratory disease in China.一种在中国与人类呼吸道疾病相关的新型冠状病毒。
Nature. 2020 Mar;579(7798):265-269. doi: 10.1038/s41586-020-2008-3. Epub 2020 Feb 3.
2
Pooled-testing procedures for screening high volume clinical specimens in heterogeneous populations.用于在异质人群中筛选大量临床标本的 pooled-testing 程序。
Stat Med. 2012 Nov 30;31(27):3261-8. doi: 10.1002/sim.5334. Epub 2012 Mar 13.
3
Detection of acute infections during HIV testing in North Carolina.北卡罗来纳州艾滋病病毒检测期间急性感染的检测
N Engl J Med. 2005 May 5;352(18):1873-83. doi: 10.1056/NEJMoa042291.
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