[木瓜蛋白酶在水含量极低的有机溶剂中催化肽的合成]
[Synthesis of peptides catalyzed by papain in organic solvents with minimal water content].
作者信息
Mitin Iu V, Schellenberger F
出版信息
Bioorg Khim. 1988 Jan;14(1):5-9.
PMID:3382433
Abstract
Synthesis of a model peptide, Z-Ala-Val-OBut, catalyzed by papain in acetonitrile containing 0-5% water was studied. Z-Ala-OCH2CONH2 was used as a carboxyl component. The optimal concentration of water was found to be 0.3-0.5%. Under these conditions the reaction rate is 50 to 100 times lower than in water but the yield of the peptide can be as high as 99.5%. Effect of reagent concentrations and temperature on the reaction was also studied.
摘要
研究了在含0 - 5%水的乙腈中木瓜蛋白酶催化合成模型肽Z - Ala - Val - OBut的过程。使用Z - Ala - OCH2CONH2作为羧基组分。发现水的最佳浓度为0.3 - 0.5%。在这些条件下，反应速率比在水中低50至100倍，但肽的产率可高达99.5%。还研究了试剂浓度和温度对反应的影响。
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