Gololobov M Iu, Schellenberger U, Schellenberger F, Jakubke H D, Svedas V K
Biokhimiia. 1990 Feb;55(2):338-45.
The effect of temperature on the kinetics of papain-catalyzed peptide synthesis was studied. A characteristic feature of this process is that the peptide synthesis is accompanied both by the hydrolysis of the synthesized bond and by the further elongation of the peptide chain. These phenomena yield the maximum on the time dependence of the first synthetic product. A decrease in temperature is an effective way to increase the yield of this substance which reflects the increase in the nucleophil reactivity resulting from the temperature decrease. Moreover, the latter diminishes the contribution of secondary enzymatic reactions such as further hydrolysis of the reaction product and polypeptide chain elongation.
研究了温度对木瓜蛋白酶催化肽合成动力学的影响。该过程的一个特征是，肽合成既伴随着合成键的水解，也伴随着肽链的进一步延长。这些现象导致第一个合成产物的时间依赖性出现最大值。降低温度是提高该物质产量的有效方法，这反映了温度降低导致亲核反应性增加。此外，后者减少了诸如反应产物的进一步水解和多肽链延长等二级酶促反应的贡献。
