Živković Ivana, Šavikin Katarina, Živković Jelena, Zdunić Gordana, Janković Teodora, Lazić Dejan, Radin Dragoslava
Institute for Vegetable Crops, 71 Karadjordjeva, Smederevska Palanka, Serbia.
Institute for Medicinal Plants Research "Dr Josif Pančić", 1 Tadeuša Košćuška Street, Belgrade, 11000, Serbia.
Plant Foods Hum Nutr. 2021 Jun;76(2):203-209. doi: 10.1007/s11130-021-00895-7. Epub 2021 Apr 7.
Human noroviruses (HuNoV) are the dominant cause of viral gastroenteritis in all age groups worldwide. In this study, we investigated the effects of pomegranate peel extract (PPE) on the reduction of HuNoV in different food models, on surfaces of fresh produce (green onion and cherry tomato), in low-fat milk, and simulated gastrointestinal fluids. The antiviral efficacy of PPE against HuNoV was evaluated by quantifying the number of residual virus genomes using a quantitative reverse transcription PCR (qRT-PCR) assay. Pomegranate peel, considered as a waste product of industrial processing, is known for beneficial health effects and broad antimicrobial activity due to the high content of phenolic compounds and tannins. PPE showed significant antiviral properties against HuNoV both in phosphate-buffered saline (PBS) and simulated gastric fluid. The reduction of HuNoV by pomegranate juice was lower than with PPE, which could be attributed to the lower content of antimicrobial compounds. A pretreatment of cherry tomato and green onion surfaces with PPE significantly reduced the amount of HuNoV particles that adhered to those surfaces during subsequent virus suspension treatment. A detrimental effect of PPE on HuNoV structure was confirmed by transmission electron microscopy. Our results indicate that PPE is a natural antiviral agent effective against food-borne noroviruses.
人诺如病毒（HuNoV）是全球所有年龄组病毒性肠胃炎的主要病因。在本研究中，我们调查了石榴皮提取物（PPE）在不同食品模型、新鲜农产品（葱和樱桃番茄）表面、低脂牛奶和模拟胃肠液中对HuNoV的减少作用。通过使用定量逆转录PCR（qRT-PCR）测定法对残留病毒基因组数量进行定量，评估了PPE对HuNoV的抗病毒效果。石榴皮被认为是工业加工的废品，因其酚类化合物和单宁含量高而具有有益健康的作用和广泛的抗菌活性。PPE在磷酸盐缓冲盐水（PBS）和模拟胃液中均对HuNoV显示出显著的抗病毒特性。石榴汁对HuNoV的减少作用低于PPE，这可能归因于抗菌化合物含量较低。用PPE对樱桃番茄和葱表面进行预处理，可显著减少后续病毒悬浮液处理过程中附着在这些表面的HuNoV颗粒数量。通过透射电子显微镜证实了PPE对HuNoV结构有损害作用。我们的结果表明，PPE是一种对食源性诺如病毒有效的天然抗病毒剂。
