Bouvet O M, Grimont P A
Unité des Entérobactéries, Unité INSERM 199, Institut Pasteur, Paris.
Ann Inst Pasteur Microbiol. 1988 Jan-Feb;139(1):59-77. doi: 10.1016/0769-2609(88)90097-x.
Extracellular D-glucose oxidation by 5 enterobacterial species was studied with the purpose of selecting conditions useful for taxonomic studies. Extracellular production of gluconate from 14C-glucose by bacterial cells was evidenced by DEAE-cellulose paper chromatography. Escherichia coli oxidized glucose only when pyrroloquinoline quinone (PQQ) was added, whereas Serratia marcescens, Yersinia frederiksenii, Erwinia cypripedii and Cedecea lapagei oxidized D-glucose without added PQQ. 2-Deoxyglucose was found to be an excellent non-metabolized analogue of D-glucose in oxidation experiments. D-glucose oxidation was inhibited by KCN, p-chloromercuribenzoic acid and carbonyl cyanide m-chlorophenylhydrazone; and activated by p-benzoquinone. Iodoacetate had no action. Comparative cellulose thin-layer chromatography including 2-ketogluconate and 2,5-diketogluconate (produced by Janthinobacterium lividum) as standards, showed that gluconate was oxidized to 2-ketogluconate by S. marcescens and E. cypripedii, and 2-ketogluconate was oxidized to 2,5-diketogluconate by E. cypripedii. The diversity of D-glucose oxidation products in the Enterobacteriaceae could have some taxonomic applications.
为了选择有助于分类学研究的条件，对5种肠杆菌属细菌的细胞外D-葡萄糖氧化进行了研究。通过DEAE-纤维素纸色谱法证明了细菌细胞从14C-葡萄糖中细胞外产生葡萄糖酸盐。只有添加吡咯并喹啉醌（PQQ）时，大肠杆菌才会氧化葡萄糖，而粘质沙雷氏菌、费氏耶尔森菌、兰氏欧文氏菌和拉氏西地西菌在不添加PQQ的情况下就能氧化D-葡萄糖。在氧化实验中，发现2-脱氧葡萄糖是D-葡萄糖的一种极好的非代谢类似物。D-葡萄糖氧化受到氰化钾、对氯汞苯甲酸和羰基氰化物间氯苯腙的抑制；并被对苯醌激活。碘乙酸没有作用。以2-酮葡萄糖酸盐和2,5-二酮葡萄糖酸盐（由青霉产黄杆菌产生）为标准品的比较纤维素薄层色谱显示，粘质沙雷氏菌和兰氏欧文氏菌将葡萄糖酸盐氧化为2-酮葡萄糖酸盐，兰氏欧文氏菌将2-酮葡萄糖酸盐氧化为2,5-二酮葡萄糖酸盐。肠杆菌科中D-葡萄糖氧化产物的多样性可能具有一些分类学应用价值。
