Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
Department of Biomedical Sciences, Chang Gung University, 259, Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, 333, Taiwan.
Geroscience. 2021 Aug;43(4):1863-1875. doi: 10.1007/s11357-021-00360-y. Epub 2021 Mar 31.
Despite the identical genomic context, trophocytes and oenocytes in worker bees exhibit aging-related phenotypes, in contrast to the longevity phenotypes in queen bees. To explore this phenomenon at the molecular level, we evaluated the age-associated transcriptomes of trophocytes and oenocytes in worker bees and queen bees using high-throughput RNA-sequencing technology (RNA-seq). The results showed that (i) while gene expression profiles were different between worker and queen bees, they remained similar between young and old counterparts; (ii) worker bees express a high proportion of low-abundance genes, whereas queen bee transcriptomes display a high proportion of moderate-expression genes; (iii) genes were upregulated to a greater extent in queen bees vs. worker bees; and (iv) distinct aging-related and longevity-related candidate genes were found in worker and queen bees. These results provide new insights into the cellular aging and longevity of trophocytes and oenocytes in honey bees. Identification of aging-associated biomarker genes also constitutes a basis for translational research of aging in higher organisms.
尽管基因组环境相同，但工蜂的滋养细胞和卵巢细胞表现出与衰老相关的表型，而蜂王则表现出长寿表型。为了在分子水平上探索这一现象，我们使用高通量 RNA 测序技术 (RNA-seq) 评估了工蜂和蜂王中滋养细胞和卵巢细胞的与年龄相关的转录组。结果表明：（i）虽然工蜂和蜂王之间的基因表达谱不同，但在年轻和年老对应物之间仍然相似；（ii）工蜂表达大量低丰度基因，而蜂王转录组则显示出大量中等表达水平的基因；（iii）与工蜂相比，蜂王中基因的上调程度更大；（iv）在工蜂和蜂王中发现了不同的与衰老相关和长寿相关的候选基因。这些结果为研究蜜蜂中滋养细胞和卵巢细胞的细胞衰老和长寿提供了新的视角。鉴定与衰老相关的生物标志物基因也为高等生物衰老的转化研究奠定了基础。
