Hsu Chin-Yuan, Hu Ting-Hui
Department of Biomedical Sciences, College of Medicine, Chang Gung University, 259, Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, 333, Taiwan,
Biogerontology. 2014 Aug;15(4):389-400. doi: 10.1007/s10522-014-9509-0. Epub 2014 Jun 28.
Queen honeybees (Apis mellifera) have much longer lifespans than worker bees. Energy-regulated molecules in the trophocytes and fat cells of workers during aging have been determined, but are unknown in queen bees. In the present study, energy-regulated molecules were evaluated in the trophocytes and fat cells of young and old queen bees. Adenosine monophosphate-activated protein kinase α2 (AMPK-α2), phosphorylated AMPK-α2 (pAMPK-α2), and cAMP-specific phosphodiesterases activity increased with aging. The pAMPK-α2/AMPK-α2 ratio and AMPK activity; adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) concentrations; the ADP/ATP ratio and the AMP/ATP ratio; the cyclic adenosine monophosphate concentration; forkhead box protein O expression; Silent information regulator T1 (SirT1) expression and activity; and peroxisome proliferator-activated receptor-α (PPAR-α) expression were not significantly different between young and old queen bees. These results show that energy-regulated molecules maintain a youthful status in the trophocytes and fat cells of queen bees during aging. These cells seem to have longevity-promoting mechanisms and may clarify the secret of longevity in queen bees.
蜂王（意大利蜜蜂）的寿命比工蜂长得多。人们已经确定了工蜂衰老过程中营养细胞和脂肪细胞中的能量调节分子，但蜂王中的情况尚不清楚。在本研究中，对年轻和年老蜂王的营养细胞和脂肪细胞中的能量调节分子进行了评估。随着衰老，单磷酸腺苷激活蛋白激酶α2（AMPK-α2）、磷酸化AMPK-α2（pAMPK-α2）和环磷酸腺苷特异性磷酸二酯酶活性增加。年轻和年老蜂王之间的pAMPK-α2/AMPK-α2比值和AMPK活性；三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和单磷酸腺苷（AMP）浓度；ADP/ATP比值和AMP/ATP比值；环磷酸腺苷浓度；叉头框蛋白O表达；沉默信息调节因子T1（SirT1）表达和活性；以及过氧化物酶体增殖物激活受体-α（PPAR-α）表达均无显著差异。这些结果表明，能量调节分子在蜂王衰老过程中能使营养细胞和脂肪细胞维持年轻状态。这些细胞似乎具有促进长寿的机制，可能会揭示蜂王长寿的秘密。
