Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, PR China.
Shandong Runde Biotechnology Co., Ltd., Tai'an 271000, PR China.
Bioresour Technol. 2021 Jul;332:125071. doi: 10.1016/j.biortech.2021.125071. Epub 2021 Mar 31.
Bioprocess, a biocatalysis-based technology, is becoming popular in many research fields and widely applied in industrial manufacturing. However, low bioconversion, low productivity, and high costs during industrial processes are usually the limitation in bioprocess. Therefore, many biocatalyst strategies have been developed to meet these challenges in recent years. In this review, we firstly discuss protein engineering strategies, which are emerged for improving the biocatalysis activity of biocatalysts. Then, we summarize metabolic engineering strategies that are promoting the development of microbial cell factories. Next, we illustrate the necessity of using the combining strategy of protein engineering and metabolic engineering for efficient biocatalysts. Lastly, future perspectives about the development and application of novel biocatalyst strategies are discussed. This review provides theoretical guidance for the development of efficient, sustainable, and economical bioprocesses mediated by novel biocatalysts.
生物工艺学是一种基于生物催化的技术，在许多研究领域中变得越来越流行，并广泛应用于工业制造。然而，在工业过程中，低生物转化、低生产力和高成本通常是生物工艺学的限制因素。因此，近年来已经开发了许多生物催化剂策略来应对这些挑战。在本文中，我们首先讨论了蛋白质工程策略，该策略的出现是为了提高生物催化剂的生物催化活性。然后，我们总结了代谢工程策略，这些策略促进了微生物细胞工厂的发展。接下来，我们说明了使用蛋白质工程和代谢工程相结合的策略对于高效生物催化剂的必要性。最后，讨论了新型生物催化剂策略的发展和应用的未来展望。本文综述为新型生物催化剂介导的高效、可持续和经济的生物工艺学的发展提供了理论指导。
