Fukui Hiroto, Shimoda Hiroshi, Kadekaru Sho, Henmi Chizuka, Une Yumi
Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoino-oka, Imabari, Ehime 794-8555, Japan.
Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, Yamaguchi 753-8515, Japan.
J Vet Med Sci. 2021 May 17;83(5):841-845. doi: 10.1292/jvms.21-0007. Epub 2021 Apr 8.
Twenty-three of 42 European rabbits (Oryctolagus cuniculus), belonging to the same rabbit colony, died in March 2020 (55% mortality) in Chiba prefecture, Japan. The disease course was extremely acute without indicators of death or hemorrhage. Necropsy revealed liver swelling, discoloration, cloudiness and fragility, and pulmonary edema. Histologically, severe hepatocellular necrosis (mainly peripheral) and intra-glomerular capillary hyalin thrombi were observed. On molecular-biological examination, reverse transcription polymerase chain reaction analysis of RNA from tissues detected a rabbit hemorrhagic disease virus, confirmed as a RHDV-2 VP60 fragment, which shared 99.42% nucleotide identity with the homologous fragment of RHDV-2 German isolate by nucleotide sequence analysis. This report shows the outbreak of rabbit hemorrhagic disease caused by RHDV-2, an emerging infectious disease, in Japan.
2020年3月，日本千叶县一个兔群中的42只欧洲兔（穴兔）中有23只死亡（死亡率55%）。病程极为急性，无死亡或出血迹象。尸检发现肝脏肿大、变色、混浊和脆弱，以及肺水肿。组织学上，观察到严重的肝细胞坏死（主要是周边）和肾小球内毛细血管透明血栓。分子生物学检查显示，对组织RNA进行逆转录聚合酶链反应分析检测到一种兔出血性疾病病毒，经确认为RHDV-2 VP60片段，通过核苷酸序列分析，其与RHDV-2德国分离株的同源片段具有99.42%的核苷酸同一性。本报告显示了日本出现由新兴传染病RHDV-2引起的兔出血性疾病疫情。
