Shirzad Shima, Neamati Ali, Vafaee Farzaneh, Ghazavi Hamed
Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, I.R. Iran.
Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, I.R. Iran.
Res Pharm Sci. 2020 Nov 27;15(6):571-582. doi: 10.4103/1735-5362.301342. eCollection 2020 Dec.
Venenum Bufonis is a Chinese traditional medicine produced from the glandular secretions of toads that contain biogenic amines, which have anti-inflammatory properties. The present study aimed to examine the effect of secretions (BVS) on anxiety and depression-like behavior and hippocampal senile plaques volume in an animal model of Alzheimer's disease (AD).
Thirty-eight male Wistar rats were used. AD was induced by amyloid-beta (Aβ) (10 μg/2 μL, intracerebroventricular injection, icv) and then BVS at 20, 40, and 80 mg/kg were injected intraperitoneally (ip) in six equal intervals over 21 days. Anxiety and depression-like behavior were assessed using behavioral tests including open field test (OFT), elevated plus maze (EPM), and forced swimming test (FST) 21 days after the surgery. The volume of senile plaques was assessed based on the Cavalieri principle.
FINDINGS/RESULTS: Results of the OFT showed that the central crossing number and the time in the AD group were significantly decreased compared to the sham group ( < 0.01 and < 0.001, respectively). Also, the values of these two parameters significantly increased in the AD + BVS80 group than the AD group ( < 0.05 and < 0.001, respectively). The time spent in the closed arm in the EPM dramatically increased in the AD group compared to the sham group ( < 0.05) and significantly decreased in the AD + BVS80 group compared to the AD group ( < 0.05). Results of the FST indicated that immobility time had a reduction in the AD + BVS20 ( < 0.01), AD + BVS40, and AD + BVS80 groups compared to the AD group ( < 0.001). The volume of senile plaques in the hippocampus showed a reduction in the treatment groups in comparison with the AD group ( < 0.001 for all).
Results revealed that BVS injection could improve symptoms of anxiety and depression and decrease senile plaques in the hippocampus in an animal model of AD.
蟾酥是一种由蟾蜍的腺体分泌物制成的中药，其含有具有抗炎特性的生物胺。本研究旨在探讨蟾酥分泌物（BVS）对阿尔茨海默病（AD）动物模型中焦虑和抑郁样行为以及海马老年斑体积的影响。
使用38只雄性Wistar大鼠。通过脑室内注射β-淀粉样蛋白（Aβ）（10μg/2μL）诱导AD，然后在21天内以六个相等的间隔腹腔注射20、40和80mg/kg的BVS。术后21天，使用包括旷场试验（OFT）、高架十字迷宫（EPM）和强迫游泳试验（FST）在内的行为测试评估焦虑和抑郁样行为。基于卡瓦列里原理评估老年斑的体积。
OFT结果显示，与假手术组相比，AD组的中央穿越次数和时间显著减少（分别为P<0.01和P<0.001）。此外，AD + BVS80组中这两个参数的值比AD组显著增加（分别为P<0.05和P<0.001）。与假手术组相比，AD组在EPM中封闭臂所花费的时间显著增加（P<0.05），与AD组相比，AD + BVS80组显著减少（P<0.05）。FST结果表明，与AD组相比，AD + BVS20组（P<0.01）、AD + BVS40组和AD + BVS80组的不动时间减少（P<0.001）。与AD组相比，治疗组海马中老年斑的体积减少（所有P<0.001）。
结果显示，在AD动物模型中，注射BVS可改善焦虑和抑郁症状，并减少海马中的老年斑。
