Valdes Julian, Samoluk Sergio Sebastian, Abdala Cristian Simón, Baldo Diego, Seijo Guillermo
Instituto de Botánica del Nordeste (UNNE-CONICET), Corrientes Capital, Corrientes, Argentina.
Unidad ejecutora Lillo (CONICET), Facultad de Ciencias Naturales e Instituto Miguel Lillo (IML), Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
PeerJ. 2021 Mar 22;9:e10677. doi: 10.7717/peerj.10677. eCollection 2021.
is the most specious genus of the Squamata lizards in South America, presenting exceptional evolutionary radiation and speciation patterns. This recent diversification complicates the formal taxonomic treatment and the phylogenetic analyses of this group, causing relationships among species to remain controversial. Here we used Next-Generation Sequencing to do a comparative analysis of the structure and organization of the complete mitochondrial genomes of three differently related species of and with different reproductive strategies and ploidy levels. The annotated mitochondrial genomes of ca. 17 kb are the first for the Liolaemidae family. Despite the high levels of sequence similarity among the three mitochondrial genomes over most of their lengths, the comparative analyses revealed variations at the stop codons of the protein coding genes and the structure of the tRNAs among species. The presence of a non-canonical dihydrouridine loop is a novelty for the pleurodonts iguanians. But the highest level of variability was observed in two repetitive sequences of the control region, which were responsible for most of the length heterogeneity of the mitochondrial genomes. These tandem repeats may be useful markers to analyze relationships of closely related species of and related genera and to conduct population and phylogenetic studies.
是南美洲有鳞目蜥蜴中最似是而非的属，呈现出异常的进化辐射和物种形成模式。这种最近的多样化使该类群的正式分类处理和系统发育分析变得复杂，导致物种间的关系仍存在争议。在这里，我们使用下一代测序技术对三种不同亲缘关系、具有不同繁殖策略和倍性水平的物种的完整线粒体基因组的结构和组织进行了比较分析。约17 kb的注释线粒体基因组是鬣蜥科的首个此类基因组。尽管三个线粒体基因组在其大部分长度上具有高度的序列相似性，但比较分析揭示了物种间蛋白质编码基因的终止密码子和tRNA结构的差异。非典型二氢尿嘧啶环的存在是侧生齿鬣蜥亚目的一个新特征。但在控制区的两个重复序列中观察到了最高水平的变异性，这两个重复序列导致了线粒体基因组的大部分长度异质性。这些串联重复序列可能是分析该属及相关属的近缘物种关系以及进行种群和系统发育研究的有用标记。
