骨髓增生异常综合征骨髓单个核细胞暴露于可溶性CD40配体后mRNA肿瘤坏死因子α（TNFα）的表达及蛋白质TNFα水平
Expression of mRNA TNFα and level of protein TNFα after exposure sCD40L in bone marrow mononuclear cells of myelodysplastic syndromes.
作者信息
Iriani Anggraini, Setiabudy Rahayuningsih D, Kresno Siti B, Sudoyo Aru W, Bardosono Saptawati, Rachman Andhika, Harahap Alida R, Arief Mansyur
机构信息
Department of Clinical Pathology, Yarsi University, Jakarta, Indonesia.
Department of Clinical Pathology, University of Indonesia, Jakarta, Indonesia.
出版信息
Stem Cell Investig. 2021 Mar 29;8:6. doi: 10.21037/sci-2020-025. eCollection 2021.
BACKGROUND
Cytopenia is the primary phenomenon in myelodysplastic syndrome (MDS) amidst hypercellular bone marrow. The soluble CD40 ligand (sCD40L) is considered as a cytokine that can trigger synthesis of tumor necrosis factor α (TNFα) that promotes apoptosis. The objective of this study is to prove that recombinant human sCD40L (rh-sCD40L) exposure on bone marrow mononuclear cells (BMMC) MDS increases TNFα expression at mRNA level and at protein level.
METHODS
BMMC from MDS patients whom diagnosed and classified using the WHO 2008 criteria, were exposed to rh-sCD40L and antiCD40L. The expressions of TNFα mRNAs were quantified by qRT-PCR, level of TNFα were measured using the ELISA method.
RESULTS
Exposure of rh-sCD40L significantly increased the expression of TNFα mRNA. The similar exposure also significantly increased the level of TNFα compared to controls. TNFα mRNA expression on BMMC in MDS samples exposed to rh-sCD40L is 3.32 times compared to TNFα mRNA expression without exposure. level of TNFα in supernatant media exposed to rh-sCD40L in MDS samples was higher than that of control samples which were 44.44 and 4.85 pg/mL, P=0.018.
CONCLUSIONS
The sCD40L plays a role in increasing the synthesis of TNFα in mRNA level and protein level in BMMC MDS.
背景
血细胞减少是骨髓增生异常综合征（MDS）骨髓细胞增多时的主要现象。可溶性CD40配体（sCD40L）被认为是一种细胞因子，可触发促进细胞凋亡的肿瘤坏死因子α（TNFα）的合成。本研究的目的是证明重组人sCD40L（rh-sCD40L）作用于MDS患者的骨髓单个核细胞（BMMC），可使TNFα在mRNA水平和蛋白水平的表达增加。
方法
采用世界卫生组织2008年标准诊断和分类的MDS患者的BMMC，分别用rh-sCD40L和抗CD40L处理。通过qRT-PCR定量检测TNFα mRNA的表达，采用ELISA法检测TNFα水平。
结果
rh-sCD40L处理显著增加了TNFα mRNA的表达。与对照组相比，同样的处理也显著提高了TNFα水平。与未处理的MDS样本BMMC中的TNFα mRNA表达相比，rh-sCD40L处理后的表达是其3.32倍。MDS样本中rh-sCD40L处理的上清液中TNFα水平高于对照样本，分别为44.44和4.85 pg/mL，P=0.018。
结论
sCD40L在提高MDS患者BMMC中TNFα的mRNA水平和蛋白水平合成方面发挥作用。
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