Avan Meltem, Palacıoğlu Gülsüm, Aksoy Cemre, Kaya Rıza, Bayraktar Harun, Katırcıoğlu Yakup Zekai, Maden Salih
Department of Plant Protection, Faculty of Agriculture, Ankara University, 06110, Ankara, Turkey.
Sugar Institute, Etimesgut, Ankara, Turkey.
Curr Microbiol. 2021 May;78(5):1939-1948. doi: 10.1007/s00284-021-02470-4. Epub 2021 Apr 7.
During 2015-2017 growing seasons, seventy-one isolates of Rhizoctonia spp. were obtained from the samples collected from the sugar beet fields of Konya province, which is one of the most important sugar beet growing areas of Turkey. Anastomosis groups (AGs) of Rhizoctonia spp. isolates were determined by hyphal conjugation with the tester strains and sequence analysis of the rDNA ITS region. The obtained data confirmed the species identity of 61 isolates out of 71 as R. solani (AG-2-2-IIIB, AG-4-(HGI, HGII, HGIII), AG-5, AG-11) and the remaining 10 isolates as binucleate Rhizoctonia (AG-K, AG-A). Pathogenicity tests revealed that AG-2-2-IIIB, AG-4-(HGI, HGII, HGIII) and AG-K isolates were highly virulent on sugar beet. The disease severity of 71 isolates varied between 13 and 100%. Based on the virulence, the isolates formed four categories; (i) 11 isolates: non-pathogenic, (ii) 15 isolates: low virulent, (iii) 6 isolates: moderately virulent and (iv) 39 isolates: highly virulent. To our knowledge, the AGs of AG-4-(HGI, HGII, HGIII), AG-11, AG-A are first reports on sugar beet in Turkey and the AG-5, AG-11 and AG-K are first AG groups for Konya region.
在2015 - 2017年生长季期间，从土耳其最重要的甜菜种植区之一科尼亚省的甜菜田采集的样本中获得了71株丝核菌属分离株。通过与测试菌株进行菌丝融合以及对rDNA ITS区域进行序列分析来确定丝核菌属分离株的融合群（AGs）。所获数据证实，71株分离株中有61株的物种鉴定为立枯丝核菌（AG - 2 - 2 - IIIB、AG - 4 -（HGI、HGII、HGIII）、AG - 5、AG - 11），其余10株为双核丝核菌（AG - K、AG - A）。致病性测试表明，AG - 2 - 2 - IIIB、AG - 4 -（HGI、HGII、HGIII）和AG - K分离株对甜菜具有高致病性。71株分离株的病情严重程度在13%至100%之间。根据致病性，分离株形成了四类：（i）11株：无致病性；（ii）15株：低毒力；（iii）6株：中等毒力；（iv）39株：高毒力。据我们所知，AG - 4 -（HGI、HGII、HGIII）、AG - 11、AG - A的融合群是土耳其甜菜上的首次报道，AG - 5、AG - 11和AG - K是科尼亚地区的首次融合群报道。
