Folkesson R, Monstein H J, Geijer T, Påhlman S, Nilsson K, Terenius L
Department of Pharmacology, Uppsala University, Sweden.
Brain Res. 1988 Apr;427(2):147-54. doi: 10.1016/0169-328x(88)90060-5.
Several human tumour cell lines were screened for secretion of proenkephalin-derived peptides with an antiserum directed to its N-terminus, Met-enkephalin-Arg6,Phe7 and for proopiomelanocortin-derived peptides with an antiserum to beta-endorphin. The neuroblastoma SK-N-MC cell line secreted Met-enkephalin-Arg6,Phe7-immunoreactive peptides in relatively high amounts into the culture medium, although processing was not complete and there was no evidence for free Met-enkephalin-Arg6,Phe7. Gene expression was confirmed by the presence of proenkephalin mRNA and proenkephalin-derived polypeptides in extracts of the SK-N-MC cells and also in the neuroblastoma SH-SY5Y cell line. In the latter cells, however, the expression was approximately 3 times lower, there was less processing of proenkephalin and no evidence for secretion.
使用针对前脑啡肽N端、甲硫氨酸脑啡肽-精氨酸6-苯丙氨酸7的抗血清，筛选了几种人类肿瘤细胞系，以检测其是否分泌前脑啡肽衍生肽；同时使用针对β-内啡肽的抗血清，筛选其是否分泌阿片促黑激素皮质素原衍生肽。神经母细胞瘤SK-N-MC细胞系向培养基中分泌了相对大量的甲硫氨酸脑啡肽-精氨酸6-苯丙氨酸7免疫反应性肽，尽管加工过程并不完整，且没有游离甲硫氨酸脑啡肽-精氨酸6-苯丙氨酸7的证据。通过在SK-N-MC细胞提取物以及神经母细胞瘤SH-SY5Y细胞系中存在前脑啡肽mRNA和前脑啡肽衍生多肽，证实了基因表达。然而，在后一种细胞中，表达量约低3倍，前脑啡肽的加工较少，且没有分泌的证据。
