Shivers B D, Harlan R E, Romano G J, Howells R D, Pfaff D W
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6221-5. doi: 10.1073/pnas.83.16.6221.
The cellular locations of proenkephalin mRNA have been determined for the caudate-putamen and cerebellar cortex of the rat brain by in situ hybridization. In the caudate-putamen, more than half of the neurons express the proenkephalin gene. Morphologically, they are medium-sized cells that may represent projection neurons. In the cerebellar cortex, proenkephalin mRNA is present in a subpopulation of neurons in the granule layer that appear to be Golgi cells--i.e., inhibitory interneurons. The presence of [Met]enkephalin, a pentapeptide derived from proenkephalin, in these two brain areas is consistent with a synthetic role for this mRNA and implicates proenkephalin gene expression in the control of motor function.
通过原位杂交确定了大鼠脑尾状核-壳核和小脑皮质中前脑啡肽原mRNA的细胞定位。在尾状核-壳核中，超过半数的神经元表达前脑啡肽原基因。从形态学上看，它们是中等大小的细胞，可能代表投射神经元。在小脑皮质中，前脑啡肽原mRNA存在于颗粒层中一部分似乎是高尔基细胞（即抑制性中间神经元）的神经元中。这两个脑区中存在源自前脑啡肽原的五肽[甲硫]脑啡肽，这与该mRNA的合成作用一致，并表明前脑啡肽原基因表达参与运动功能的控制。
