接种 COVID-19 恢复期血浆供者疫苗后，中和抗体和刺突特异性抗体呈指数增长。
Exponential increase in neutralizing and spike specific antibodies following vaccination of COVID-19 convalescent plasma donors.
机构信息
Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa.
Department of Pathology, University of Iowa, Iowa City, Iowa.
出版信息
Transfusion. 2021 Jul;61(7):2099-2106. doi: 10.1111/trf.16401. Epub 2021 Apr 15.
BACKGROUND
With the recent approval of COVID-19 vaccines, recovered COVID-19 subjects who are vaccinated may be ideal candidates to donate COVID-19 convalescent plasma (CCP).
CASE SERIES
Eleven recovered COVID-19 patients were screened to donate CCP. All had molecularly confirmed COVID-19, and all but one were antibody positive by chemiluminescence immunoassay (DiaSorin) prior to vaccination. All were tested again for antibodies 11-21 days after they were vaccinated (Pfizer/Moderna). All showed dramatic increases (~50-fold) in spike-specific antibody levels and had at least a 20-fold increase in the IC50 neutralizing antibody titer based on plaque reduction neutralization testing (PRNT). The spike-specific antibody levels following vaccination were significantly higher than those seen in any non-vaccinated COVID-19 subjects tested to date at our facility.
CONCLUSION
Spike-specific and neutralizing antibodies demonstrated dramatic increases following a single vaccination after COVID-19 infection, which significantly exceeded values seen with COVID-19 infection alone. Recovered COVID-19 subjects who are vaccinated may make ideal candidates for CCP donation.
背景
随着最近 COVID-19 疫苗的批准，接种疫苗的 COVID-19 康复者可能是捐赠 COVID-19 恢复期血浆（CCP）的理想人选。
病例系列
筛选了 11 名康复的 COVID-19 患者进行 CCP 捐赠。所有患者均经分子检测确诊 COVID-19，在接种疫苗前，除 1 例外均通过化学发光免疫分析（DiaSorin）呈抗体阳性。所有患者在接种疫苗后 11-21 天再次接受抗体检测（辉瑞/莫德纳）。所有患者的刺突蛋白特异性抗体水平均显著升高（~50 倍），根据蚀斑减少中和试验（PRNT），中和抗体的 IC50 滴度至少增加了 20 倍。接种疫苗后的刺突蛋白特异性抗体水平明显高于本机构迄今检测到的任何未接种 COVID-19 患者的水平。
结论
在 COVID-19 感染后单次接种疫苗后，刺突蛋白特异性和中和抗体显著增加，显著超过 COVID-19 单独感染时的水平。接种疫苗的 COVID-19 康复者可能是 CCP 捐赠的理想人选。
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