Leon Judith, Merrill Anna E, Rogers Kai, Kurt Julie, Dempewolf Spencer, Ehlers Alexandra, Jackson J Brooks, Knudson C Michael
Department of Pathology, University of Iowa Hospitals & Clinics, Iowa City, IA, United States.
Department of Pathology, University of Iowa Hospitals & Clinics, Iowa City, IA, United States.
Transfus Apher Sci. 2022 Apr;61(2):103326. doi: 10.1016/j.transci.2021.103326. Epub 2021 Nov 23.
Vaccination has been shown to stimulate remarkably high antibody levels in donors who have recovered from COVID-19. Our objective was to measure patient antibody levels before and after transfusion with COVID-19 Convalescent Plasma (CCP) and compare the antibody levels following transfusion of CCP from vaccinated and nonvaccinated donors. Plasma samples before and after transfusion were obtained from 25 recipients of CCP and COVID-19 antibody levels measured. Factors that effect changes in antibody levels were examined. In the 21 patients who received CCP from nonvaccinated donors, modest increases in antibody levels were observed. Patients who received two units were more likely to seroconvert than those receiving just one unit. The strongest predictor of changes in patient antibody level was the CCP dose, calculated by the unit volume multiplied by the donor antibody level. Using patient plasma volume and donor antibody level, the post-transfusion antibody level could be predicted with reasonable accuracy(R> 0.90). In contrast, the 4 patients who received CCP from vaccinated donors all had dramatic increases in antibody levels following transfusion of a single unit. In this subset of recipients, antibody levels observed after transfusion of CCP were comparable to those seen in donors who had fully recovered from COVID-19. If available, CCP from vaccinated donors with very high antibody levels should be used. One unit of CCP from vaccinated donors increases patient antibody levels much more than 1 or 2 units of CCP from unvaccinated donors.
疫苗接种已被证明能在从新冠病毒感染中康复的献血者体内刺激产生非常高的抗体水平。我们的目标是测量输注新冠康复者血浆（CCP）前后患者的抗体水平，并比较输注来自接种疫苗和未接种疫苗献血者的CCP后的抗体水平。从25名CCP接受者身上获取输血前后的血浆样本，并测量新冠病毒抗体水平。研究了影响抗体水平变化的因素。在21名接受未接种疫苗献血者的CCP的患者中，观察到抗体水平有适度升高。接受两单位CCP的患者比仅接受一单位的患者更有可能发生血清转化。患者抗体水平变化的最强预测因素是CCP剂量，通过单位体积乘以献血者抗体水平来计算。利用患者血浆体积和献血者抗体水平，可以相当准确地预测输血后的抗体水平（R>0.90）。相比之下，4名接受接种疫苗献血者的CCP的患者在输注一单位后抗体水平均有显著升高。在这一亚组接受者中，输注CCP后观察到的抗体水平与从新冠病毒感染中完全康复的献血者体内的抗体水平相当。如果有可用的CCP，应使用来自抗体水平非常高的接种疫苗献血者的CCP。一单位来自接种疫苗献血者的CCP比一单位或两单位来自未接种疫苗献血者的CCP更能提高患者的抗体水平。
