Department of Microtechnology and Nanoscience, Chalmers University of Technology, 9 Kemivägen, Gothenborg, 412 96, Sweden.
Department of Macromolecular Science, Collaborative Innovation Center of Polymers and Polymer Composites, Fudan University, 2005 Songhu Road, Shanghai, 200433, China.
ChemistryOpen. 2021 Jul;10(7):666-671. doi: 10.1002/open.202000200. Epub 2021 Apr 7.
During the last decades, there has been growing interest in using therapeutic messager RNA (mRNA) together with drug delivery systems. Naked, unformulated mRNA is, however, unable to cross the cell membrane and is susceptible to degradation. Here we use graphene quantum dots (GQDs) functionalized with polyethyleneimine (PEI) as a novel mRNA delivery system. Our results show that these modified GQDs can be used to deliver intact and functional mRNA to Huh-7 hepatocarcinoma cells at low doses and, that the GQDs are not toxic, although cellular toxicity is a problem for these first-generation modified particles. Functionalized GQDs represent a potentially interesting delivery system that is easy to manufacture, stable and effective.
在过去的几十年中，人们越来越感兴趣地将治疗性信使 RNA（mRNA）与药物传递系统一起使用。然而，裸露的、未经配制的 mRNA 无法穿过细胞膜，并且容易降解。在这里，我们使用经过聚乙烯亚胺（PEI）功能化的石墨烯量子点（GQDs）作为新型 mRNA 传递系统。我们的结果表明，这些修饰的 GQDs 可以在低剂量下将完整且功能正常的 mRNA 递送到 Huh-7 肝癌细胞中，并且 GQDs 没有毒性，尽管细胞毒性是这些第一代修饰颗粒的一个问题。功能化的 GQDs 代表了一种潜在的有趣的传递系统，它易于制造、稳定且有效。
