Centro Internacional de Entrenamiento e Investigaciones Médicas, CIDEIM, Cali, Valle del Cauca, Colombia.
Clinical & Experimental Sciences, Faculty of Medicine, University of Southampton, General Hospital Southampton, SO16 6YD, Southampton, UK.
Bioanalysis. 2021 Apr;13(8):655-667. doi: 10.4155/bio-2021-0013. Epub 2021 Apr 8.
A high-throughput method using inductively coupled plasma mass spectrometry (ICP-MS) was developed and validated for the quantitative analysis of antimony in human plasma and peripheral blood mononuclear cells from patients with cutaneous leishmaniasis undergoing treatment with meglumine antimoniate. Antimony was digested in clinical samples with 1% tetramethylammonium hydroxide/1% EDTA and indium was used as internal standard. Accuracy, precision and stability were evaluated. Taking the lower limit of quantitation to be the lowest validation concentration with precision and accuracy within 20%, the current assay was successfully validated from 25 to 10000 ng/ml for antimony in human plasma and peripheral blood mononuclear cells. This protocol will serve as a baseline for future analytical designs, aiming to provide a reference method to allow inter-study comparisons.
建立并验证了一种使用电感耦合等离子体质谱法（ICP-MS）的高通量方法，用于定量分析接受葡甲胺锑治疗的皮肤利什曼病患者的人血浆和外周血单个核细胞中的锑。采用 1%四甲基氢氧化铵/1% EDTA 对临床样本进行消化，并用铟作为内标。对准确性、精密度和稳定性进行了评估。将定量下限定义为精密度和准确度在 20%以内的最低验证浓度，目前的检测方法已成功验证人血浆和外周血单个核细胞中锑的浓度范围为 25 至 10000ng/ml。该方案将作为未来分析设计的基准，旨在提供一种参考方法以实现研究间的比较。
