Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.
Clin Infect Dis. 2021 Dec 16;73(12):2326-2328. doi: 10.1093/cid/ciab063.
An ultra-sensitive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid antigen assay (S-PLEX, MesoScale Diagnostics) was evaluated in 250 retrospective and 200 prospective upper respiratory specimens. In samples with cycle threshold <35, there was 95%-98% positive and 93%-96% negative percent agreement with reverse transcription-polymerase chain reaction. S-PLEX may provide a high-throughput alternative to nucleic acid-based testing for coronavirus disease 2019 (COVID-19) diagnosis.
一种超灵敏的严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）核衣壳抗原检测试剂盒（S-PLEX，MesoScale Diagnostics）对 250 份回顾性和 200 份前瞻性上呼吸道标本进行了评估。在循环阈值<35 的样本中，与逆转录-聚合酶链反应（RT-PCR）相比，该检测试剂盒的阳性符合率为 95%-98%，阴性符合率为 93%-96%。S-PLEX 可能为新型冠状病毒病（COVID-19）诊断提供一种高通量的核酸检测替代方法。
