College of Animal Science and Technology, Shihezi University, Xinjiang, China.
Glycoconj J. 2018 Apr;35(2):243-253. doi: 10.1007/s10719-018-9817-8. Epub 2018 Feb 20.
Selenium (Se) is an essential trace element for human beings and many other forms of life. Organic selenium from natural foods has greater bioavailability and is safer than inorganic selenium species. In this article, the structural properties and antioxidant activities of a Glycyrrhiza uralensis polysaccharide (GUP) after selenylation modification were investigated. The GUP was extracted by water decoction and ethanol precipitation and purified via protein elimination using the trichloroacetic acid method and column chromatography. The purified product was subsequently modified by the nitric acid-sodium selenite (HNO-NaSeO) method. The selenized GUP (SeGUP) product was characterized by Fourier transform-infrared (FT-IR) spectroscopy, and its thermal stability, particle size, and antioxidant activities were investigated. FT-IR analysis indicated that the selenium in SeGUP existed mainly as O-Se-O. The thermal stability and particle size of SeGUP differed significantly from those of GUP. Moreover, compared to GUP, SeGUP exhibited greater antioxidant activities in vitro and in vivo. These results indicate that selenylation modification significantly enhances the antioxidant activity of SeGUP, increasing its potential for application as an antioxidant. Graphical abstract ᅟ.
硒（Se）是人类和许多其他生命形式的必需微量元素。天然食物中的有机硒具有更高的生物利用度，比无机硒更安全。本文研究了甘草多糖（GUP）硒化修饰后的结构性质和抗氧化活性。GUP 通过水提法和乙醇沉淀提取，并使用三氯乙酸法和柱层析通过去除蛋白质进行纯化。然后，通过硝酸-亚硒酸钠（HNO-NaSeO）法对纯化产物进行修饰。通过傅里叶变换-红外（FT-IR）光谱对硒化 GUP（SeGUP）产物进行了表征，并对其热稳定性、粒径和抗氧化活性进行了研究。FT-IR 分析表明，SeGUP 中的硒主要以 O-Se-O 的形式存在。SeGUP 的热稳定性和粒径与 GUP 有显著差异。此外，与 GUP 相比，SeGUP 在体外和体内均表现出更强的抗氧化活性。这些结果表明，硒化修饰显著提高了 SeGUP 的抗氧化活性，增加了其作为抗氧化剂的应用潜力。
